Electrochemical treatment of human KB cells in vitro.
Electrochemical treatment of human KB cells in vitro.
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DOI：
10.1002/(sici)1521-186x(1999)20:1
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发表时间：
1999
影响因子：
1.9
通讯作者：
Yun Yen;Jian Ren Li;Bing Sen Zhou;Fernando Rojas;Jonathan Yu;Chung-Kwang Chou
中科院分区：
文献类型：
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作者：
Yun Yen;Jian Ren Li;Bing Sen Zhou;Fernando Rojas;Jonathan Yu;Chung-Kwang Chou
癌症的电化学治疗（ECT）是一种有前途的新方法，通过将直流电输送到肿瘤组织中来诱导肿瘤消退。本研究的目的是评估 ECT 对人类癌细胞的有效性，并调查影响 ECT 的因素。还探讨了 ECT 在癌症治疗中的生物学机制。使用人 KB 细胞，发现 ECT 通过使用 0.3 库仑 (C)/ml（1.5 C，5 ml 培养基；3 V，400 microA，62.5 分钟）延迟细胞生长。集落形成测定的结果清楚地表明，增加 ECT 剂量会降低肿瘤细胞的存活率。使用亚甲蓝测定的细胞毒性研究确定，对于培养物中的 2.5 x 10(5) 个细胞，1D50 为 0.68 C/ml。对于 0.6 C/ml 的固定剂量（5 ml 中 3 C），使用更高的电流和更短的处理时间会导致更好的细胞存活。因此，时间是一个重要因素。当细胞浓度改变时，细胞浓度越高，存活率越高。胸苷掺入测定表明，随着 ECT 剂量的增加，掺入 DNA 中的 [3H] 胸苷的量减少。用 1 C/ml（5 C 于 5 ml；3 V，400 microA，持续 208.4 分钟）处理后，阳极的 pH 值降至 4.53，阴极的 pH 值升至 10.46。这些结果表明ECT能够有效地在体外杀死人KB细胞，并且毒性作用与电荷、电流和处理时间有关。 pH改变对细胞的影响是ECT的机制之一。
Electrochemical treatment (ECT) of cancer is a promising new method by which direct current is delivered into tumor tissue to induce tumor regression. The purpose of this study is to evaluate the effectiveness of ECT on human cancer cells and to investigate the factors that affect ECT. The biological mechanisms of ECT in cancer treatment were also explored. Using human KB cells, ECT was found to delay cell growth by using 0.3 coulombs (C)/ml (1.5 C in 5 ml of culture medium; 3 V, 400 microA for 62.5 min). From the results of a colony-forming assay, it was clearly demonstrated that increasing the ECT dose decreases tumor cell survival. A cytotoxicity study, in which a methylene blue assay was used, determined that, for 2.5 x 10(5) cells in culture, the 1D50 was 0.68 C/ml. For a fixed dose of 0.6 C/ml (3 C in 5 ml), using higher current and shorter treatment time resulted in better cell survival. Time, therefore, is an important factor. When cell concentration was altered, the survival was higher for increased cell concentrations. A thymidine incorporation assay indicated that the amount of [3H]thymidine incorporated into DNA decreased as the ECT dose increased. After treatment with 1 C/ml (5 C in 5 ml; 3 V, 400 microA for 208.4 min), pH at the anode decreased to 4.53 and at the cathode increased to 10.46. These results indicate that ECT is effective for killing human KB cells in vitro and that the toxicity effect is related to charge, current, and treatment time. The effect of pH alteration on cells is one of the mechanisms of ECT.