Comprehensive analysis of HER2 expression and gene amplification in gastric cancers using immunohistochemistry and in situ hybridization: which scoring system should we use?
Comprehensive analysis of HER2 expression and gene amplification in gastric cancers using immunohistochemistry and in situ hybridization: which scoring system should we use?
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DOI：
10.1016/j.humpath.2011.05.019
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发表时间：
2012-03-01
期刊：
影响因子：
3.3
通讯作者：
Kang, Yoon-Koo
中科院分区：
文献类型：
--
作者：
Park, Young Soo;Hwang, Hee Sang;Kang, Yoon-Koo
据报道，HER 2表达在胃癌和乳腺癌中是不同的，并且最近提出了胃癌评分系统（GCSS）。我们使用GCSS和乳腺癌评分系统（BCSS）研究了HER 2蛋白表达，并将其与HER 2基因扩增相关。通过分析使用来自每个病例的2个不同核心构建的组织微阵列，在1091个病例中评估HER 2状态。使用多克隆（HercepTest）和单克隆（Pathway）抗体进行免疫组织化学（IHC），并通过BCSS和GCSS对结果进行评分。所有病例均采用全自动双色银染增强原位杂交（SISH）检测基因扩增，并与590例荧光原位杂交（FISH）结果进行对照。使用多克隆和单克隆抗体的IHC结果之间的一致性较高（kappa = 0.785）。双色SISH与FISH检测结果一致性较高（kappa = 1.918）。两种抗体中，GCSS检测SISH阳性的敏感性明显高于BCSS（多克隆，P = .003;单克隆，P < .001），但BCSS的特异性高于GCSS（多克隆，P =.104;单克隆，P <.001）。
It has been reported that HER2 expression is different in gastric and breast cancers, and a gastric cancer scoring system (GCSS) has recently been suggested. We investigated HER2 protein expression using GCSS and a breast cancer scoring system (BCSS) and correlated it with HER2 gene amplification. HER2 status was evaluated in 1091 cases by analyzing tissue microarrays constructed using 2 different cores from each case. Polyclonal (HercepTest) and monoclonal (Pathway) antibodies were used for immunohistochemistry (IHC), and results were scored by BCSS and GCSS. Gene amplification was evaluated by automated dual-color silver-enhanced in situ hybridization (SISH) in all cases and correlated with the results from fluorescence in situ hybridization (FISH) in 590 eases. The concordance between the IHC results using polyclonal and monoclonal antibodies was high (kappa = 0.785). The results of dual-color SISH and FISH showed very high concordance as well (kappa = (1.918). GCSS was significantly more sensitive for detecting SISH positivity than was BCSS in both antibodies (polyclonal, P = .003; monoclonal, P < .001), but specificity was higher in BCSS than GCSS (polyclonal, P =.104; monoclonal, P