Imaging of single molecule diffusion
Imaging of single molecule diffusion
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DOI：
10.1073/pnas.93.7.2926
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发表时间：
1996-04-02
影响因子：
11.1
通讯作者：
Schindler, H
中科院分区：
文献类型：
--
作者：
Schmidt, T;Schutz, GJ;Schindler, H
近年来，通过显微镜和光谱学，在单个原子和分子水平上的观察成为可能。单荧光分子的成像已经实现，但迄今为止一直局限于分子在固定状态下，在这里，我们提供的方法可视化的单个荧光分子的运动，它适用于成像的单分子的扩散路径的磷脂膜通过使用磷脂携带一个罗丹明染料分子。对于这种方法，荧光显微镜进行的灵敏度，使单一的荧光分子照明只有5毫秒是可分辨的信号/噪声比为28。重复照明允许直接观察的扩散运动的单个分子的位置精度为30纳米，这种能力在生物科学中具有迷人的潜力，例如，相关的生物功能的细胞膜的运动，空间组织，和化学计量的单个组件。
In recent years observations at the level of individual atoms and molecules became possible by microscopy and spectroscopy. Imaging of single fluorescence molecules has been achieved but has so far been restricted to molecules in the immobile state, Here we provide methodology for visualization of the motion of individual fluorescent molecules, It is applied to imaging of the diffusional path of single molecules in a phospholipid membrane by using phospholipids carrying one rhodamine dye molecule. For this methodology, fluorescence microscopy was carried to a sensitivity so that single fluorescent molecules illuminated for only 5 ms were resolvable at a signal/noise ratio of 28. Repeated illuminations permitted direct observation of the diffusional motion of individual molecules with a positional accuracy of 30 nm, Such capability has fascinating potentials in bioscience-for example, to correlate biological functions of cell membranes with movements, spatial organization, and stoichiometries of individual components.