In vivo stimulation of prolactin secretion in the rat by thyrotropin releasing factor, related peptides and hypothalamic extracts.
In vivo stimulation of prolactin secretion in the rat by thyrotropin releasing factor, related peptides and hypothalamic extracts.
复制标题
促甲状腺素释放因子、相关肽和下丘脑提取物体内刺激大鼠催乳素分泌。
作者：
C. Rivier;W. Vale
pGlu-His-Pro-NH2 (TRF) is shown to be a potent stimulator of PRL secretion in the estrogen-progesterone-pretreated male (EP) rat. A series of structural analogues of pGlu-His-Pro-NH2 were found to have comparable potencies to stimulate both PRL and TSH in the EP rat. Using pGlu-His-Pro-NH2 as a standard, the amount of PRL releasing activity in various hypothalamic extracts, as determined in the EP rat, is in agreement with the amount of TSH releasing activity as measured by a mouse TRF assay, but is not in agreement with the amount of TSH releasing activity determined by measurement of plasma TSH levels in the EP rat as the assay animal. This discrepancy is best explained by a differential sensitivity of the two methods, to TSH release inhibiting substances such as somatostatin. The PRL releasing activity as well as the TSH releasing activity of hypothalamic extracts is destroyed by incubation in rat serum. These results suggest that the hypothalamic substance(s) responsible for PRL releasing activity pos...
