THE PRIMARY STRUCTURE AND GENETIC ORGANIZATION OF THE BOVINE PAPILLOMAVIRUS TYPE-1 GENOME
THE PRIMARY STRUCTURE AND GENETIC ORGANIZATION OF THE BOVINE PAPILLOMAVIRUS TYPE-1 GENOME
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DOI：
10.1038/299529a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
SEEBURG, PH
中科院分区：
文献类型：
--
作者：
CHEN, EY;HOWLEY, PM;SEEBURG, PH
测定了牛乳头瘤病毒1型（BPV-1）双链环状DNA的全序列。结合已知的病毒转录数据分析该序列为确定乳头瘤病毒基因组的组织提供了基础。所有主要的开放阅读框都位于同一条DNA链上。在BPV转化的细胞中转录的区域在所有3个翻译框中都含有开放框，而在生产性感染的牛纤维乳头状瘤中转录的区域的特征在于由单个翻译终止密码子分隔的2个大的开放阅读框。启动子和多聚腺苷酸化位点的诊断序列的定位表明剪接参与病毒mRNA的生物合成。序列比较表明牛乳头瘤病毒的基因组结构和SV 40-多瘤病毒亚组成员的基因组结构是不同的，这表明这两组病毒在进化上是无关的。
The complete nucleotide sequence of the double-stranded circular DNA of bovine papillomavirus type 1 (BPV-1) was determined. Analysis of this sequence in conjunction with known transcriptional data for the virus provides a basis for determining the organization of the papillomavirus genome. All the major open reading frames are located on the same DNA strand. The region transcribed in BPV-transformed cells contains open frames in all 3 translational frames whereas the region transcribed in productivity infected bovine fibropapillomas is characterized by 2 large open reading frames partitioned by a single translational stop codon. The localization of sequences diagnostic of promoters and polyadenylation sites suggests that splicing is involved in the biosynthesis of the viral mRNA. A sequence comparison indicates the genomic organization of the bovine papillomavirus and that of the members of the SV40-polyomavirus subgroup to be distinct, suggesting that these 2 groups of viruses are evolutionarily unrelated.