Novel screen methodologies for identification of new microbial metabolites with pharmacological activity.
Novel screen methodologies for identification of new microbial metabolites with pharmacological activity.
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用于鉴定具有药理活性的新微生物代谢物的新筛选方法。
DOI：
10.1007/bfb0102297
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
L. J. Nisbet
中科院分区：
文献类型：
--
作者：
D. Hill;S. Wrigley;L. J. Nisbet
微生物继续为发现具有新生物活性的化合物提供重要的化学多样性来源。最近发现的微生物代谢产物使用检测具有潜在药理学效用的化合物的分析进行调查，发现代表了广泛的各种生物体产生的广泛的结构类型。用于筛选微生物过程产生的样品的测定必须是稳健的、灵敏的和特异性的，并且能够在来自许多来源的潜在干扰的背景之上操作。目前使用的发现测定分为三个主要类别：基于细胞的测定、受体-配体相互作用测定和酶抑制测定。这些检测方法的使用趋势和适用于微生物样品筛选的检测技术的新发展，特别是高通量筛选环境进行了检查。为了使微生物筛选成为新药先导的竞争途径，所涉及的学科必须被设计成一个无缝集成的过程，以快速提供具有所需生物学特性的新型化合物。
Micro-organisms continue to provide an important source of chemical diversity for the discovery of compounds with new biological activities. Microbial metabolites discovered recently using assays to detect compounds with potential pharmacological utility are surveyed and found to represent an extensive range of structural types produced by a wide variety of organisms. Assays used for screening samples produced by microbial processes must be robust, sensitive and specific and able to operate above a background of potential interferences from a number of sources. Discovery assays currently in use fall into three main categories cell-based, receptor-ligand interaction and enzyme inhibition assays. Trends in the use of these assays and new developments in assay technology applicable to the screening of microbial samples are examined with particular reference to the high throughput screening environment. For microbial screening to be a competitive route to new drug leads, the disciplines involved must be engineered into a seamlessly integrated process to deliver novel compounds with the required biological properties rapidly.
影响因子：
2.9
作者：
ABE, M;HARPEL, JG;RIFKIN, DB
通讯作者：
RIFKIN, DB