Complete mitochondrial genome of Palpita hypohomalia (Lepidoptera: Pyraloidea: Crambidae) and its phylogenetic implications
Complete mitochondrial genome of Palpita hypohomalia (Lepidoptera: Pyraloidea: Crambidae) and its phylogenetic implications
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触肢虫（鳞翅目：螟总科：螟科）的完整线粒体基因组及其系统发育意义
DOI：
10.14411/eje.2018.070
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发表时间：
2018-01-01
影响因子：
1.3
通讯作者：
Liu, Xiaomeng
中科院分区：
文献类型：
--
作者：
Yang, Mingsheng;Shi, Shusen;Liu, Xiaomeng
对吡咯烷属物种 Palpitahypohomalia 的完整线粒体基因组进行了测序和分析。该线粒体基因组呈环状，长15,280 bp，包括37个典型的后生动物线粒体基因（13个蛋白质编码基因、2个核糖体RNA基因、22个转移RNA基因）和一个富含A + T的区域。核苷酸组成高度偏向 A + T 核苷酸 (81.6%)。所有 13 个蛋白质编码基因 (PCG) 均以规范起始密码子 ATN 起始，cox1 除外，它是 CGA。典型的终止密码子 TAA 出现在大多数 PCG 中，而 nad2 和 cox2 分别显示 TAG 和不完整的终止密码子 T。所有 tRNA 都具有典型的三叶草结构，除了 trnS1 (AGN) 缺乏二氢尿苷 (DHU) 臂。比较线粒体基因组分析表明，atp8和atp6之间的基序“ATGATAA”以及trnS2和nad1之间的基序“ATACTAA”普遍存在于鳞翅目有丝分裂基因组中。此外，“ATAG”和随后的多聚T结构以及富含A的3'末端在鳞翅目有丝分裂基因组的富含A+T的区域中是保守的。基于我们的 37 个线粒体基因数据集的系统发育分析得出了与螟总科相同的拓扑结构，并且与之前基于多个核基因的研究所恢复的拓扑结构基本相同。在之前对 Crambidae 的研究中，Evergestinae 与 Glaphyriinae 是同义的。本研究首次阐明了它们与线粒体基因组数据的密切关系。
The complete mitochondrial genome of a pyraloid species, Palpita hypohomalia, was sequenced and analyzed. This mitochondrial genome is circular, 15,280 bp long, and includes 37 typical metazoan mitochondrial genes (13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes) and an A + T-rich region. Nucleotide composition is highly biased toward A + T nucleotides (81.6%). All 13 protein-coding genes (PCGs) initiate with the canonical start codon ATN, except for cox1 which is CGA. The typical stop codon TAA occurs in most PCGs, while nad2 and cox2 show TAG and an incomplete termination codon T, respectively. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) which lacks the dihydrouridine (DHU) arm. Comparative mitochondrial genome analysis showed that the motif "ATGATAA" between atp8 and atp6, and the motif "ATACTAA" between trnS2 and nad1 were commonly present in lepidopteran mitogenomes. Furthermore, the "ATAG" and subsequent poly-T structure, and the A-rich 3' end were conserved in the A + T-rich regions of lepidopteran mitogenomes. Phylogenetic analyses based on our dataset of 37 mitochondrial genes yielded identical topology for the Pyraloidea, and is generally identical with that recovered by a previous study based on multiple nuclear genes. In a previous study of the Crambidae, the Evergestinae was synonymized with Glaphyriinae; the present study is the first to clarify their close relationship with mitogenome data.