Quantitation of normal metabolite concentrations in six brain regions by in-vivoH-MR spectroscopy.
Quantitation of normal metabolite concentrations in six brain regions by in-vivoH-MR spectroscopy.
复制标题
DOI：
10.4103/0971-6203.62128
复制
发表时间：
2010-07
影响因子：
0.9
通讯作者：
Erbetta A
中科院分区：
文献类型：
--
作者：
Minati L;Aquino D;Bruzzone MG;Erbetta A
本研究检测了28名正常受试者样本中1.5 T下体内1H-磁共振波谱（1H-MRS）可见的脑代谢物浓度。尝试使用开源软件定量肌醇化合物、胆碱单位、总肌酸和N-乙酰基部分。六个脑区被认为是：额叶和顶叶白色物质，内侧颞叶，丘脑，脑桥和小脑。使用组织水作为内部参考和使用外部参考推导绝对浓度;还计算了代谢产物相对于肌酸的信号强度比。与信号强度比相比，绝对浓度（内部参比）的个体间变异性较小。发现所有代谢物的浓度存在显著的区域变异性，表明不同脑区需要单独的标准值。在本研究中获得的值可以作为参考，在未来的研究中，提供了相同的方法是遵循;它证实，尽管在过去的尝试不成功，较小的变异系数确实可以通过绝对定量获得。
This study examined the concentrations of brain metabolites visible to in-vivo 1H-Magnetic Resonance Spectroscopy (1H-MRS) at 1.5 T in a sample of 28 normal subjects. Quantitation was attempted for inositol compounds, choline units, total creatine and N-acetyl moieties, using open-source software. Six brain regions were considered: frontal and parietal white matter, medial temporal lobe, thalamus, pons and cerebellum. Absolute concentrations were derived using tissue water as an internal reference and using an external reference; metabolite signal intensity ratios with respect to creatine were also calculated. The inter-individual variability was smaller for absolute concentrations (internal reference) as compared to that for signal intensity ratios. Significant regional variability in concentration was found for all metabolites, indicating that separate normative values are needed for different brain regions. The values obtained in this study can be used as reference in future studies, provided the same methodology is followed; it is confirmed that despite unsuccessful attempts in the past, smaller coefficients of variation can indeed be obtained through absolute quantification.