Electrophysiological methods for Caenorhabditis elegans neurobiology.
Electrophysiological methods for Caenorhabditis elegans neurobiology.
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DOI：
10.1016/b978-0-12-394620-1.00014-x
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发表时间：
2012
影响因子：
--
通讯作者：
Richmond, Janet E.
中科院分区：
文献类型：
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作者：
Goodman, Miriam B.;Lindsay, Theodore H.;Lockery, Shawn R.;Richmond, Janet E.
膜片钳电生理学是对C.线虫C. elegans电生理学在十年以来首次发表这项技术。今天，即使不是所有的电生理学研究都可以在大型动物标本中进行，也可以在C.优美的本章有两个主要目标。第一个是向广大观众介绍可用于C中神经元、肌肉和突触的膜片钳分析的许多技术。优雅的第二部分是对膜片钳技术进行方法学介绍。elegans神经元和体壁肌肉在体内，包括新兴的方法与突触后记录耦合的光遗传学刺激。我们还提出了细胞内和突触后离子电流的样本，可以在C。神经和肌肉。
Patch-clamp electrophysiology is the technique of choice for the biophysical analysis of the function of nerve, muscle, and synapse in C. elegans nematodes. Considerable technical progress has been made in C. elegans electrophysiology in the decade since the initial publication of this technique. Today, most, if not all electrophysiological studies that can be done in larger animal preparations can also be done in C. elegans. This chapter has two main goals. The first is to present to a broad audience the many techniques available for patch-clamp analysis of neurons, muscles, and synapses in C. elegans. The second is to provide a methodological introduction to the techniques for patch-clamping C. elegans neurons and body-wall muscles in vivo, including emerging methods for optogenetic stimulation coupled with post-synaptic recording. We also present samples of the cell-intrinsic and post-synaptic ionic currents that can be measured in C. elegans nerve and muscle.