Sperm motility modulated by Trpv1 regulates zebrafish fertilization
Sperm motility modulated by Trpv1 regulates zebrafish fertilization
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Trpv1调节精子活力调节斑马鱼受精
DOI：
10.1016/j.theriogenology.2020.03.032
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发表时间：
2020-07-15
期刊：
影响因子：
2.8
通讯作者：
Zeng, Xuhui
中科院分区：
文献类型：
--
作者：
Chen, Ying;Wang, Hantsing;Zeng, Xuhui
通过响应环境和细胞内刺激，离子通道在精子功能调节中起着关键作用。尽管离子通道在雄性生殖中的重要性在许多物种中引起了越来越多的关注，但对斑马鱼精子中离子通道的了解仍然有限。在这里，我们表明，斑马鱼精子活力可以抑制一般的钙通道阻滞剂，而不是一般的钾通道阻滞剂。进一步的研究发现，精子活力不仅被瞬时受体电位香草酸通道亚型1（Trpv 1）的拮抗剂抑制，而且还被其激动剂恢复，这表明Trpv 1在斑马鱼精子中的功能存在。因此，Trpv 1拮抗剂抑制精子活力可降低体外受精率。Western blot和免疫荧光分析证明Trpv 1主要分布在斑马鱼精子的颈部和尾部区域。此外，Trpv 1的拮抗剂和激动剂对trpv 1（-/-）斑马鱼精子的活力均无影响。据我们所知，这是为数不多的显示离子通道在调节斑马鱼精子功能中的重要性的研究之一，可以丰富我们对斑马鱼雄性生殖生理的理解，并为鱼类育种和精子冷冻保存提供新的调控靶点。(C)2020爱思唯尔公司All rights reserved.
By responding to environmental and intracellular stimuli, ion channels play critical roles in sperm function regulation. Although the importance of ion channel in male reproduction has drawn increasing attention in many species, the knowledge about ion channels in zebrafish sperm is limited. Here, we show zebrafish sperm motility could be suppressed by general calcium channel blockers rather than by general potassium channel blockers. Further investigation found that sperm motility was not only suppressed by antagonist for the transient receptor potential vanilloid channel, subtype 1 (Trpv1), but also restored by its agonist, suggesting functional presence of Trpv1 in zebrafish spermatozoa. As a consequence, the suppression of sperm motility by Trpv1 antagonist could reduce in vitro fertilization rate. Western blot and immunofluorescence analysis proved that Trpv1 was mainly distributed in the neck and tail regions of zebrafish sperm. Additionally, neither antagonist nor agonist of Trpv1 exhibited effect on the motility of trpv1(-/-) zebrafish sperm. To our knowledge, this is one of the limited studies showing the importance of ion channels in regulating zebrafish sperm function, and may enrich our understanding on male reproductive physiology of zebrafish and offer novel regulatory target for fish breeding and sperm cryopreservation. (C) 2020 Elsevier Inc. All rights reserved.