A new chlorophyll d-containing cyanobacterium: evidence for niche adaptation in the genus Acaryochloris
A new chlorophyll d-containing cyanobacterium: evidence for niche adaptation in the genus Acaryochloris
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DOI：
10.1038/ismej.2010.67
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发表时间：
2010-11-01
期刊：
影响因子：
11
通讯作者：
Hess, Wolfgang R.
中科院分区：
文献类型：
--
作者：
Mohr, Remus;Voss, Bjoern;Hess, Wolfgang R.
叶绿素 d 是一种光合色素，基于化学分析，直到最近才被认为广泛存在于海洋和湖泊环境中。然而，携带这种色素的生物体的多样性尚不清楚。迄今为止，单细胞蓝细菌 Acaryocholis marina 是唯一一种使用叶绿素 d 作为主要感光色素的生物体。在这项研究中，我们描述了一种具有大量叶绿素 d 的新蓝藻，它是从大堡礁赫伦岛（南纬 23 度 26' 31.2 '' ，东经 151 度 54' 50.4 ''）周围水域中分离出来的。 16S 核糖体 RNA 与先前描述的 A. marina 的两个分离株有 2% 的差异，这两个分离株是从帕劳群岛（太平洋）和索尔顿湖（加利福尼亚州）周围的水域中分离出来的，表明它属于 Acaryophoris 属的不同分支。基于Illumina技术对其基因组进行总体序列分析，得到871个重叠群，累积长度为8 371 965 nt。他们的分析揭示了与螨绿藻相关的典型特征，例如叶绿素结合蛋白的扩展基因家族。然而，与A. marina MBIC11017相比，观察到明显的遗传、形态和生理差异。在较低光强度下达到光饱和，叶绿素 d/a 比率随光强度变化较小，并且缺乏藻胆蛋白藻蓝蛋白，这表明 Acaryo绿藻属蓝藻存在于不同的生态型中。这些数据将 Acaryoloris 描述为一种适应环境的蓝藻，并表明值得进行更严格的尝试来分离、培养和分析含叶绿素 d 的蓝藻，以了解这些生物体的生态生理学。 ISME 期刊 (2010) 4, 1456-1469； doi：10.1038/ismej.2010.67； 2010 年 5 月 27 日在线发布
Chlorophyll d is a photosynthetic pigment that, based on chemical analyses, has only recently been recognized to be widespread in oceanic and lacustrine environments. However, the diversity of organisms harbouring this pigment is not known. Until now, the unicellular cyanobacterium Acaryochloris marina is the only characterized organism that uses chlorophyll d as a major photopigment. In this study we describe a new cyanobacterium possessing a high amount of chlorophyll d, which was isolated from waters around Heron Island, Great Barrier Reef (23 degrees 26' 31.2 '' S, 151 degrees 54' 50.4 '' E). The 16S ribosomal RNA is 2% divergent from the two previously described isolates of A. marina, which were isolated from waters around the Palau islands (Pacific Ocean) and the Salton Sea lake (California), suggesting that it belongs to a different clade within the genus Acaryochloris. An overview sequence analysis of its genome based on Illumina technology yielded 871 contigs with an accumulated length of 8 371 965 nt. Their analysis revealed typical features associated with Acaryochloris, such as an extended gene family for chlorophyll-binding proteins. However, compared with A. marina MBIC11017, distinct genetic, morphological and physiological differences were observed. Light saturation is reached at lower light intensities, Chl d/a ratios are less variable with light intensity and the phycobiliprotein phycocyanin is lacking, suggesting that cyanobacteria of the genus Acaryochloris occur in distinct ecotypes. These data characterize Acaryochloris as a niche-adapted cyanobacterium and show that more rigorous attempts are worthwhile to isolate, cultivate and analyse chlorophyll d-containing cyanobacteria for understanding the ecophysiology of these organisms. The ISME Journal (2010) 4, 1456-1469; doi: 10.1038/ismej.2010.67; published online 27 May 2010