Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase.
Molecular cloning and sequence analysis of cDNA encoding human ferrochelatase.
复制标题
编码人亚铁螯合酶的 cDNA 的分子克隆和序列分析。
DOI：
10.1016/s0006-291x(05)80099-3
复制
发表时间：
1990
影响因子：
3.1
通讯作者：
Rikio Tokunaga
中科院分区：
文献类型：
--
作者：
Y. Nakahashi;S. Taketani;M. Okuda;Kyoichi Inoue;Rikio Tokunaga
用放射性标记的小鼠铁螯合酶cDNA片段进行筛选，从噬菌体λgt11的人胎盘cDNA文库中分离到编码人铁螯合酶的cDNA [EC 4.99.1.1]。该cDNA有一个1269碱基对（bp）的开放阅读框，编码423个氨基酸残基的蛋白（Mr. 47,833），在3 '非编码区和聚(a)尾部可能存在多聚腺苷化信号。氨基酸测序结果表明，该成熟蛋白由369个氨基酸残基组成（Mr. 42,158），其中推测的先导序列为54个氨基酸残基。人酶与小鼠酶的同源性为88%，与酵母酶的同源性为46%。Northern blot分析显示，K562和HepG2细胞中铁螯合酶的mrna分别为2500 bp和1600 bp。随着人类铁螯合酶全长cDNA的获得，与红细胞生成性原生卟啉症相关的分子病变可以被表征。
The cDNA encoding human ferrochelatase [EC 4.99.1.1] was isolated from a human placenta cDNA library in bacteriophage λgt11 by screening with a radiolabeled fragment of mouse ferrochelatase cDNA. The cDNA had an open reading frame of 1269 base pairs (bp) encoding a protein of 423 amino acid residues (Mr. 47,833) with alternative putative polyadenylation signals in the 3′ non-coding regions and poly (A) tails. Amino acid sequencing showed that the mature protein consists of 369 amino acid residues (Mr. 42, 158) with a putative leader sequence of 54 amino acid residues. The human enzyme showed an 88% identity to mouse enzyme and 46% to yeast enzyme. Northern blot analysis showed two mRNAs of about 2500 and 1600 bp for ferrochelatase in K562 and HepG2 cells. As full-length cDNA for human ferrochelatase is now available, molecular lesions related to erythropoietic protoporphyria can be characterized.
DOI：
10.1016/s0021-9258(18)61070-1
发表时间：
1987-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Matsudaira
通讯作者：
P. Matsudaira
DOI：
10.1016/s0021-9258(17)44247-5
发表时间：
1983-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Dailey;J. Fleming
通讯作者：
H. Dailey;J. Fleming