Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection
Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection
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DOI：
10.1016/j.vetmic.2004.01.013
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发表时间：
2004-05-20
影响因子：
3.3
通讯作者：
Hasman, H
中科院分区：
文献类型：
--
作者：
Aarestrup, FM;Hasman, H
使用 MIC 测定，测试了总共 569 种不同的细菌分离株（156 种沙门氏菌、202 种大肠杆菌、43 种金黄色葡萄球菌、38 种猪链球菌、52 种粪肠球菌、78 种屎肠球菌）对硫酸铜、苯扎氯铵、过氧化氢和氯己定的敏感性。还测试了总共 442 个分离株对甲醛的敏感性和 177 个分离株对氯化锌的敏感性。肠球菌分离株对硫酸铜形成了 MIC 的双峰分布，而其他细菌种类形成了一个大种群。否则，分离株形成一大群对不同抗菌剂敏感的群体。不同细菌种类对不同化合物的敏感性存在很大差异。一般来说，葡萄球菌对所有测试的抗菌化合物都非常敏感。沙门氏菌分离株一般对硫酸铜、苯扎氯铵和氯己定不太敏感，其次是大肠杆菌和革兰氏阳性菌。氯化锌的情况正好相反。所有分离株都对 H2O2 非常敏感，MIC 范围为 0.002 至 0.016%，对甲醛非常敏感，MIC 范围为 0.003 和 0.006%。这项研究表明，迄今为止，从牲畜中分离出来的丹麦细菌尚未或仅在有限程度上对通常用于消毒的抗菌化合物产生了耐药性。仅在肠球菌中发现获得性铜耐药性。不同细菌种类对这些化合物的内在敏感性存在很大差异，尤其是沙门氏菌本质上似乎比其他细菌种类更不易敏感，这可能会对人类健康产生影响。 (C) 2004 Elsevier B.V. 保留所有权利。
A total of 569 different bacterial isolates (156 Salmonella, 202 E. coli, 43 S. aureus, 38 S. hyicus, 52 E. faecalis, 78 E faecium) were tested for susceptibility to copper sulphate, benzalkonium chloride, hydrogen peroxide and chlorhexidine using MIC determinations. A total of 442 isolates were also tested for susceptibility to formaldehyde and 177 isolates for susceptibility to zinc chloride. Enterococcal isolates formed a bimodal distribution of MICs to copper sulphate, whereas the other bacterial species formed one large population. Otherwise the isolates formed one large population of susceptibilities to the different antimicrobial agents. Large variations were observed in the susceptibility of the different bacterial species to the different compounds. Staphylococci were in general very susceptible to all antimicrobial compounds tested. The Salmonella isolates were in general less susceptible to copper sulphate, benzalkonium chloride and chlorhexidine followed by E. coli and the Gram-positive species. The opposite was the case for zinc chloride. All isolates were very susceptible to H2O2 with MICs ranging from 0.002 to 0.016%, and to formaldehyde with MICs at 0.003 and 0.006%. This study showed that Danish bacterial isolates from livestock so far have not or have only to a limited degree developed resistance to antimicrobial compounds commonly used for disinfection. Acquired copper resistance was only found in enterococci. There were large differences in the intrinsic susceptibility of the different bacterial species to these compounds, and Salmonella especially seems intrinsically less susceptible than the other bacterial species, which might have human health implications. (C) 2004 Elsevier B.V. All rights reserved.