High levels of Gardnerella vaginalis detected with an oligonucleotide probe combined with elevated pH as a diagnostic indicator of bacterial vaginosis.
High levels of Gardnerella vaginalis detected with an oligonucleotide probe combined with elevated pH as a diagnostic indicator of bacterial vaginosis.
复制标题
使用寡核苷酸探针检测到高水平的阴道加德纳菌，并结合升高的 pH 值作为细菌性阴道病的诊断指标。
DOI：
10.1128/jcm.30.3.642-648.1992
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发表时间：
1992
影响因子：
9.4
通讯作者：
Hillier,SL
中科院分区：
文献类型：
--
作者：
Sheiness,D;Dix,K;Watanabe,S;Hillier,SL
我们展示了一种诊断细菌性阴道病 (BV) 的新方法，该方法基于使用 DNA 探针测量阴道液中阴道加德纳菌的浓度。阴道加盖氏菌实际上总是以高浓度存在于患有 BV 的女性中，但在正常女性中也经常检测到，通常浓度低于 10(7) CFU/ml 阴道液。阴道 pH 值升高是 BV 的另一个敏感指标，尽管它可能与其他情况同时发生。我们提出，只要阴道 pH 值高于 4.5，使用特定 DNA 探针对阴道加泰罗尼亚杆菌进行定量测量可以作为诊断 BV 的有用帮助。为了检验这一假设，首先通过一组标准的临床体征对 113 名女性进行了 BV 评估。收集阴道洗液，并通过定量培养分析等分试样的阴道加德纳菌浓度。这些相同样品的部分与定量标准一起固定在尼龙过滤器上。将过滤器与阴道加德纳菌 16S rRNA 特异性的放射性标记寡核苷酸一起孵育，并检查随后的放射自显影图以确定每个样品中阴道加德纳菌的水平。然后对浓度大于或等于 2 x 10(7) CFU/ml 且阴道 pH 值大于 4.5 的阴道加泰罗尼亚进行分析，以确保其与基于临床标准的诊断相符。与基于临床标准的诊断相比，该狭缝印迹分析的结果给出了 95% 的敏感性，正确分类了 43 个 BV 阳性样本中的 41 个，特异性为 99%，正确识别了 70 个 BV 阴性样本中的 69 个。
We have demonstrated a new approach to diagnosing bacterial vaginosis (BV) that is based on measuring the concentration of Gardnerella vaginalis in vaginal fluid with DNA probes. G. vaginalis is virtually always present at high concentrations in women who have BV but is also detected frequently in normal women, usually at concentrations of less than 10(7) CFU/ml of vaginal fluid. Elevated vaginal pH is another sensitive indicator of BV, although it can occur in conjunction with other conditions. We have proposed that quantitative measurements of G. vaginalis using specific DNA probes can serve as a useful aid in diagnosing BV, provided the vaginal pH is above 4.5. To test this hypothesis, a group of 113 women were first evaluated for BV by the standard set of clinical signs. Vaginal washes were collected, and aliquots were analyzed by quantitative culture for the concentration of G. vaginalis. Portions of these same samples were immobilized on nylon filters, along with standards for quantitation. The filters were incubated with a radiolabelled oligonucleotide specific for G. vaginalis 16S rRNA, and the subsequent autoradiographs were examined to determine levels of G. vaginalis in each sample. G. vaginalis at concentrations of greater than or equal to 2 x 10(7) CFU/ml and vaginal pH of greater than 4.5 were then analyzed for concurrence with the diagnoses based on clinical criteria. Results of this slot blot analysis gave a sensitivity of 95%, correctly categorizing 41 of 43 BV-positive specimens, and a specificity of 99%, correctly identifying 69 of 70 BV-negative specimens, compared with diagnosis based on clinical criteria.