Quantitative Histopathology of Stained Tissues using Color Spatial Light Interference Microscopy (cSLIM)
Quantitative Histopathology of Stained Tissues using Color Spatial Light Interference Microscopy (cSLIM)
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DOI：
10.1038/s41598-019-50143-x
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发表时间：
2019-10-11
影响因子：
4.6
通讯作者：
Popescu, Gabriel
中科院分区：
文献类型：
--
作者：
Majeed, Hassaan;Keikhosravi, Adib;Popescu, Gabriel
临床组织活检评估需要定量的疾病标志物进行诊断，最重要的是预测预后。在这些新技术中，定量相位成像（QPI）通过折射率揭示了组织的内在纳米结构，因此在组织病理学方面表现出了前景。然而，过去绝大多数QPI调查都依赖于未染色组织的成像，这破坏了既定的标本处理。在这里，我们提出彩色空间光干涉显微镜（cSLIM）作为一种新的全幻灯片成像方式，对染色组织进行干涉成像，使用颜色探测器阵列。因此，cSLIM在一次扫描中产生了组织固有相位图和病理学家熟悉的标准彩色亮场图像。我们对196例乳腺癌患者的研究结果表明，cSLIM可以从肿瘤微环境中胶原纤维的排列提供不依赖于染色的预后信息。通过数学归一化校正染色对组织相图的影响。这些特点可能会减少新的cSLIM技术临床转化的障碍。
Tissue biopsy evaluation in the clinic is in need of quantitative disease markers for diagnosis and, most importantly, prognosis. Among the new technologies, quantitative phase imaging (QPI) has demonstrated promise for histopathology because it reveals intrinsic tissue nanoarchitecture through the refractive index. However, a vast majority of past QPI investigations have relied on imaging unstained tissues, which disrupts the established specimen processing. Here we present color spatial light interference microscopy (cSLIM) as a new whole-slide imaging modality that performs interferometric imaging on stained tissue, with a color detector array. As a result, cSLIM yields in a single scan both the intrinsic tissue phase map and the standard color bright-field image, familiar to the pathologist. Our results on 196 breast cancer patients indicate that cSLIM can provide stain-independent prognostic information from the alignment of collagen fibers in the tumor microenvironment. The effects of staining on the tissue phase maps were corrected by a mathematical normalization. These characteristics are likely to reduce barriers to clinical translation for the new cSLIM technology.