Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma
Comparison of mesenchymal stem cells from bone marrow and adipose tissue for bone regeneration in a critical size defect of the sheep tibia and the influence of platelet-rich plasma
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DOI：
10.1016/j.biomaterials.2010.01.085
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Kasten, Philip
中科院分区：
文献类型：
--
作者：
Niemeyer, Philipp;Fechner, Katharina;Kasten, Philip
本研究的目的是在大型动物模型中比较骨髓间充质干细胞（BMSC）和脂肪组织干细胞（ASC）的成骨潜力，并评估富血小板血浆（PRP）对ASC成骨能力的影响。将绵羊BMSC（BMSC组）和ASC（ASC组）接种在矿化胶原海绵上并植入绵羊胫骨的临界尺寸缺损处（n = 5）每个）。在另一组中，富血小板血浆 (PRP) 与 ASC 联合使用（PRP 组）。未负载的矿化胶原蛋白（空组）作为对照（每组 n = 5）。每 2 周进行一次射线照相评估，26 周后进行组织学分析。放射照相评估显示，第 10 周时，与 ASC 组相比，以及从第 12 周起，BMSC 组的新形成骨量显着高于 EMPTY 组（均 p < 0.05）。发现 PRP 组与空对照组相比在骨形成方面的放射照相水平具有优势 (p < 0.05)，但 ASC 组则不然。组织学分析证实了放射学评估发现了类似的意义。 总之，ASC 的成骨潜力似乎不如 BMSC，但可以通过添加 PRP 部分补偿。 (C) 2010 Elsevier Ltd. 保留所有权利。
Aim of the present study was to compare the osteogenic potential of bone marrow derived mesenchymal stem cells (BMSC) and adipose-tissue derived stem cells (ASC) and to evaluate the influence of platelet-rich plasma (PRP) on the osteogenic capacity of ASC in a large animal model.Ovine BMSC (BMSC-group) and ASC (ASC-group) were seeded on mineralized collagen sponges and implanted into a critical size defect of the sheep tibia (n = 5 each). In an additional group, platelet-rich plasma (PRP) was used in combination with ASC (PRP-group). Unloaded mineralized collagen (EMPTY-group) served as control (n = 5 each).Radiographic evaluation was performed every 2 weeks, after 26 weeks histological analysis was performed. Radiographic evaluation revealed a significantly higher amount of newly formed bone in the BMSC-group compared to the ASC-group at week 10 and compared to EMPTY-group from week 12 (all p < 0.05). A superiority on radiographic level concerning bone formation of the PRP-group versus the empty control group was found (p < 0.05), but not for the ASC-group. Histological analysis confirmed radiographic evaluation finding analogous significances.In conclusion, ASC seem to be inferior to BMSC in terms of their osteogenic potential but that can partially be compensated by the addition of PRP. (C) 2010 Elsevier Ltd. All rights reserved.