Excessive amounts of mu heavy chain block B-cell development.
Excessive amounts of mu heavy chain block B-cell development.
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过量的 mu 重链会阻碍 B 细胞发育。
DOI：
10.1093/intimm/dxr049
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发表时间：
2011
影响因子：
4.4
通讯作者：
Dunnick,Wesley
中科院分区：
文献类型：
--
作者：
Zhu,Lingqiao;Chang,Cheong-Hee;Dunnick,Wesley
抗原非依赖性B细胞发育发生在取决于IG重链和轻链表达的几个阶段。我们鉴定了一种在脾脏中缺乏成熟B细胞的小鼠品系。该小鼠系携带约11个拷贝的鼠重链恒定区基因座的转基因，并且B系细胞表达过量的细胞内μ重链。在前/前B细胞转换时，骨髓中的B细胞发育失败，对具有不同拷贝数的相同转基因的其他系的检查表明，B细胞发育的缺陷随着转基因拷贝数的增加而增加。转基因（Tg）轻链沿着Tg μ重链的表达导致骨髓中B细胞发育和脾脏中B细胞的最小拯救。由于过量重链表达，原/前B细胞死亡有几种潜在机制。
Antigen-independent B-cell development occurs in several stages that depend on the expression of Ig heavy and light chain. We identified a line of mice that lacked mature B cells in the spleen. This mouse line carried approximately 11 copies of a transgene of the murine heavy chain constant region locus, and B-lineage cells expressed excessive amounts of the intracellular μ heavy chain. B-cell development failed in the bone marrow at the pro/pre B-cell transition, and examination of other lines with various copy numbers of the same transgene suggested that deficiencies in B-cell development increased with increased transgene copy number. Expression of a transgenic (Tg) light chain along with the Tg μ heavy chain led to minimal rescue of B-cell development in the bone marrow and B cells in the spleen. There are several potential mechanisms for the death of pro/pre B cells as a consequence of excess heavy chain expression.