Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity.
Pseudomonas fluorescens Filamentous Hemagglutinin, an Iron-Regulated Protein, Is an Important Virulence Factor that Modulates Bacterial Pathogenicity.
复制标题
荧光假单胞菌丝状血凝素是一种铁调节蛋白，是调节细菌致病性的重要毒力因子
DOI：
10.3389/fmicb.2016.01320
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发表时间：
2016
影响因子：
5.2
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Sun YY;Chi H;Sun L
荧光假单胞菌（Pseudomonasfluorescens）是一种常见的细菌性病原体，广泛存在于包括各种鱼类在内的水产养殖动物中。在这项研究中，我们采用蛋白质组学分析，并确定了丝状血凝素（FHA）作为铁反应蛋白分泌的TSS，致病性荧光假单胞菌分离。体外研究表明，与野生型相比，fha突变体TSSfha（i）表现出在很大程度上相似的营养生长特征，但生物膜生长和产生胞外基质的能力显著降低，（ii）没有明显的鞭毛和运动性，（iii）在与宿主细胞的附着上有缺陷，并且不能形成自聚集，（iv）在宿主血清中表现出明显降低的血凝能力和存活能力。体内感染分析表明，TSSfha在鱼组织中传播和诱导宿主死亡的能力显着减弱，并且野生型TSS产生的天然FHA的抗体阻断削弱了病原体的感染性。此外，当作为亚单位疫苗引入大菱鲆时，重组FHA引起对致死TSS攻击的显著保护。总之，这些结果首次表明荧光假单胞菌FHA是与致病性相关的多种生物过程所必需的关键毒力因子。
Pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. In this study, we employed proteomic analysis and identified filamentous hemagglutinin (FHA) as an iron-responsive protein secreted by TSS, a pathogenic P. fluorescens isolate. In vitro study showed that compared to the wild type, the fha mutant TSSfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth and producing extracellular matrix, (ii) displayed no apparent flagella and motility, (iii) was defective in the attachment to host cells and unable to form self-aggregation, (iv) displayed markedly reduced capacity of hemagglutination and surviving in host serum. In vivo infection analysis revealed that TSSfha was significantly attenuated in the ability of dissemination in fish tissues and inducing host mortality, and that antibody blocking of the natural FHA produced by the wild type TSS impaired the infectivity of the pathogen. Furthermore, when introduced into turbot as a subunit vaccine, recombinant FHA elicited a significant protection against lethal TSS challenge. Taken together, these results indicate for the first time that P. fluorescens FHA is a key virulence factor essential to multiple biological processes associated with pathogenicity.