Coordinated Bacteriocin Expression and Competence in Streptococcus pneumoniae Contributes to Genetic Adaptation through Neighbor Predation.
Coordinated Bacteriocin Expression and Competence in Streptococcus pneumoniae Contributes to Genetic Adaptation through Neighbor Predation.
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DOI：
10.1371/journal.ppat.1005413
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发表时间：
2016-02
期刊：
影响因子：
6.7
通讯作者：
Dawid S
中科院分区：
文献类型：
--
作者：
Wholey WY;Kochan TJ;Storck DN;Dawid S
尽管抗生素和疫苗的广泛使用，肺炎链球菌（肺炎球菌）仍然是人类侵袭性和粘膜疾病的持久原因。这种生物体的复原力是由于其通过从周围微生物群落吸收和整合新的遗传物质而适应的能力。DNA的摄取和重组是由一个严格调控的群体感应系统控制的，该系统由感受态刺激肽（CSP）的细胞外积累触发。在这项研究中，我们证明，CSP可以刺激生产的blp细菌素的各种阵列。这种交叉刺激通过增加细菌素信息素BlpC的产生和分泌而发生，并且需要功能性感受态调节系统。我们发现，一个高度保守的基序的操纵子编码BlpC及其转运介导的CSP上调。CSP刺激后BlpC的积累导致整个blp基因座的激活增强。使用生物膜生长的生物体作为粘膜表面上的竞争和遗传交换的模型，我们证明了细菌素分泌增强了DNA交换，这表明细菌素与竞争力的共刺激提供了适应性优势。blp和com调节途径被认为在肺炎球菌的远祖中已经分化和特化。尽管如此，这两个系统保持了一种调节连接，通过靶向裂解大量潜在的竞争对手，同时提供掺入其DNA的手段，来促进竞争和适应。细菌病原体肺炎链球菌是一种适应性很强的生物体，由于频繁摄取新的基因序列，它在抗生素和疫苗的巨大选择压力下得以生存。DNA是由S.在感受态期间，当调节途径被触发时，导致DNA摄取和整合所需的蛋白质的产生。在这项研究中，我们证明，分泌的小抗菌肽称为细菌素诱导期间的能力。这些细菌素在肺炎球菌群体中非常多样化，靶向邻近的生物体进行裂解。在感受态过程中肽的协调生产可以通过增加潜在的DNA供体库而使产生更大细菌素库的肺炎球菌菌株具有选择性优势。
Streptococcus pneumoniae (pneumococcus) has remained a persistent cause of invasive and mucosal disease in humans despite the widespread use of antibiotics and vaccines. The resilience of this organism is due to its capacity for adaptation through the uptake and incorporation of new genetic material from the surrounding microbial community. DNA uptake and recombination is controlled by a tightly regulated quorum sensing system that is triggered by the extracellular accumulation of competence stimulating peptide (CSP). In this study, we demonstrate that CSP can stimulate the production of a diverse array of blp bacteriocins. This cross stimulation occurs through increased production and secretion of the bacteriocin pheromone, BlpC, and requires a functional competence regulatory system. We show that a highly conserved motif in the promoter of the operon encoding BlpC and its transporter mediates the upregulation by CSP. The accumulation of BlpC following CSP stimulation results in augmented activation of the entire blp locus. Using biofilm-grown organisms as a model for competition and genetic exchange on the mucosal surface, we demonstrate that DNA exchange is enhanced by bacteriocin secretion suggesting that co-stimulation of bacteriocins with competence provides an adaptive advantage. The blp and com regulatory pathways are believed to have diverged and specialized in a remote ancestor of pneumococcus. Despite this, the two systems have maintained a regulatory connection that promotes competition and adaptation by targeting for lysis a wide array of potential competitors while simultaneously providing the means for incorporation of their DNA. The bacterial pathogen, Streptococcus pneumoniae, is a highly adaptable organism that has managed to survive in the face of significant selective pressure from antibiotics and vaccines due to frequent uptake of new gene sequences. DNA is acquired from the environment by S. pneumoniae during the competence state when regulatory pathways are triggered that result in the production of proteins required for DNA uptake and integration. In this study, we demonstrate that the secretion of small antimicrobial peptides called bacteriocins is induced during competence. These bacteriocins, which are quite diverse in pneumococcal populations, target neighboring organisms for lysis. The coordinated production of the peptides during competence may allow pneumococcal strains that produce a greater repertoire of bacteriocins a selective advantage by increasing the potential pool of DNA donors.