Preferential Fluid Flow in the Human Trabecular Meshwork Near Collector Channels
Preferential Fluid Flow in the Human Trabecular Meshwork Near Collector Channels
复制标题
DOI：
10.1167/iovs.08-2375
复制
发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Fautsch, Michael P.
中科院分区：
文献类型：
--
作者：
Hann, Cheryl R.;Fautsch, Michael P.
目的.为了确定人类小梁网内是否存在优先通路，检查了邻近和收集通道之间的色素和非色素区域，并分析了小梁管组织（JCT）的结构。用0.5 μ m荧光珠在10或25 mm Hg下灌注健康的整个人眼睛。从每只眼睛中解剖出色素和非色素网状组织楔（有和没有收集通道），并通过共聚焦显微镜进行检查。对收集器通道附近和收集器通道之间的珠浓度进行定量。通过光学显微镜分析在20 mm Hg下灌注的全眼收集器通道附近的JCT的配置。在25 mm Hg下灌注的眼睛中，色素区域邻近收集通道的珠子多于非色素区域（4.9% +/- 3.5% vs. 1.1% +/- 0.9%; P = 0.02）。在没有收集器通道的色素沉着区域中，珠浓度降低了5倍（4.9% +/- 3.5% vs. 0.96% +/- 0.88%; P = 0.04）。与在10 mm Hg灌注的眼睛中的相同区域相比，在25 mm Hg灌注的色素收集器通道下的珠增加了三倍。在25 mm Hg下，JCT扩张发生在收集通道下的频率高于10 mm Hg下（44% vs. 17%; P = 0.01）。与相邻收集器通道之间的JCT区域相比，收集器通道下方的JCT区域扩大（1053 +/- 424 μ m（2）vs. 571 +/- 216 μ m（2）; P < 0.001）。邻近收集器通道的有色小梁网中的珠粒水平增加表明人类小梁网中存在优先流动途径。在升高的压力下，收集器通道下的JCT区域扩展，可能是因为流体流量增加。（Invest Ophthalmol维斯科学。2009; 50：1692-1697）DOI：10.1167/iovs.08-2375
PURPOSE. To determine whether preferential pathways exist within the human trabecular meshwork, pigmented and nonpigmented regions adjacent to and between collector channels were examined, and the configuration of the juxtacanalicular tissue (JCT) was analyzed.METHODS. Healthy whole human eyes were perfused at 10 or 25 mm Hg with 0.5 mu m fluorescent beads. Tissue wedges of pigmented and nonpigmented meshwork (with and without collector channels) were dissected from each eye and examined by confocal microscopy. Bead concentration adjacent to and between collector channels was quantified. The configuration of the JCT adjacent to collector channels from whole eyes perfused at 20 mm Hg was analyzed by light microscopy.RESULTS. Eyes perfused at 25 mm Hg had more beads adjacent to collector channels in pigmented than in nonpigmented regions (4.9% +/- 3.5% vs. 1.1% +/- 0.9%; P = 0.02). In pigmented regions without collector channels, bead concentration was decreased by fivefold (4.9% +/- 3.5% vs. 0.96% +/- 0.88%; P = 0.04). Perfusion at 25 mm Hg increased beads by threefold under pigmented collector channels compared with the same regions in eyes perfused at 10 mm Hg. Expansion of the JCT occurred more often under collector channels at 25 mm Hg than at 10 mm Hg (44% vs. 17%; P = 0.01). The JCT region under collector channels was expanded compared with JCT regions between adjacent collector channels (1053 +/- 424 mu m(2) vs. 571 +/- 216 mu m(2); P < 0.001).CONCLUSIONS. Increased levels of beads in pigmented trabecular meshwork adjacent to collector channels suggest preferential flow pathways are present in human trabecular meshwork. At elevated pressure, the JCT region under collector channels is expanded, possibly because of increased fluid flow. (Invest Ophthalmol Vis Sci. 2009; 50: 1692-1697) DOI:10.1167/iovs.08-2375