Antibodies of predetermined specificity detect two retroviral oncogene products and inhibit their kinase activities.
Antibodies of predetermined specificity detect two retroviral oncogene products and inhibit their kinase activities.
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具有预定特异性的抗体可检测两种逆转录病毒癌基因产物并抑制它们的激酶活性。
DOI：
10.1073/pnas.80.5.1246
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发表时间：
1983
影响因子：
11.1
通讯作者：
Sen,A
中科院分区：
文献类型：
--
作者：
Sen,S;Houghten,RA;Sherr,CJ;Sen,A
根据癌基因 v-fesof 猫肉瘤病毒 (FeSV) 的核苷酸序列预测的寡肽通过化学合成并用于产生特异性抗体。针对位于 v-fescoding 序列羧基末端 42 个残基的 12 个氨基酸长寡肽（12 聚体）的抗血清可有效识别由 FeSV 的 Snyder-Theilen (ST) 和 Gardner-Arnstein (GA) 菌株编码的转化蛋白。该12聚体还含有10个氨基酸残基，其顺序和位置与根据禽富士南肉瘤病毒(FSV)的致癌基因v-fps的核苷酸序列预测的氨基酸残基同源。抗 12 聚体免疫沉淀来自 FSV 转化细胞的 FSV 特异性转化蛋白分子。这些抗肽抗体分子与v-fes和v-fps基因产物的结合抑制了它们相关的酪氨酸特异性蛋白激酶(EC 2.7.1.37)活性。产生针对相关癌基因产物的这种位点特异性抗血清的能力对于逆转录病毒转化蛋白及其正常细胞同源物的分子表征将是有价值的。
Oligopeptides predicted from the nucleotide sequence of the oncogene v-fesof feline sarcoma virus (FeSV) were synthesized chemically and used to generate specific antibodies. Antisera against a 12-amino-acid-long oligopeptide (12-mer) located 42 residues from the carboxyl terminus of the v-fescoding sequence efficiently recognized the transforming proteins encoded by Snyder-Theilen (ST) and Gardner-Arnstein (GA) strains of FeSV. This 12-mer also contains 10 amino acid residues homologous in order and position to those predicted from the nucleotide sequence of the oncogene v-fpsof avian Fujinami sarcoma virus (FSV). The anti-12-mer immunoprecipitated the FSV-specific transforming protein molecules from FSV-transformed cells. Binding of these antipeptide antibody molecules to the v-fes and the v-fps gene products inhibited their associated tyrosine-specific protein kinase (EC 2.7.1.37) activities. The ability to generate such site-specific antisera to the products of related oncogenes will be valuable in the molecular characterization of retroviral transforming proteins and their normal cellular homologs.