Jaagsiekte sheep retrovirus utilizes a pH-dependent endocytosis pathway for entry
Jaagsiekte sheep retrovirus utilizes a pH-dependent endocytosis pathway for entry
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DOI：
10.1128/jvi.01853-07
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发表时间：
2008-03-01
影响因子：
5.4
通讯作者：
Liu, Shan-Lu
中科院分区：
文献类型：
--
作者：
Bertrand, Pascale;Cote, Marceline;Liu, Shan-Lu
使用Moloney鼠白血病病毒假病毒携带的Jaagsiekte羊逆转录病毒（JSRV）的包膜蛋白，我们在这里报告，进入弱抑制溶酶体剂，但被深深地封锁巴弗洛霉素A1（BafA1）。动力学研究表明，JSRV进入是一个缓慢的过程，基本上被一个显性负突变的发动蛋白阻断。有趣的是，低pH脉冲克服了BafA1对JSRV感染的阻断，尽管这仅发生在病毒结合的细胞在37 ℃下预孵育时，与非常早期的进入事件一致，例如在低pH依赖性步骤发生之前需要内吞作用。此外，JSRV假病毒粒子对低pH灭活具有抵抗力。总而言之，这项研究表明，JSRV利用pH依赖性，动力蛋白相关的内吞途径进入，不同于经典的pH依赖性进入途径的水泡性口炎病毒。
Using Moloney murine leukemia virus pseudovirions bearing the envelope protein of Jaagsiekte sheep retrovirus (JSRV), we report here that entry was weakly inhibited by lysosomotropic agents but was profoundly blocked by bafilomycin A1 (BafA1). Kinetics studies revealed that JSRV entry is a slow process and was substantially blocked by a dominant-negative mutant of dynamin. Interestingly, a low-pH pulse overcame the BafA1 block to JSRV infection, although this occurred only if virus-bound cells were preincubated at 37 degrees C, consistent with a very early entry event such as endocytosis being required before the low-pH-dependent step occurs. Moreover, JSRV pseudovirions were resistant to low-pH inactivation. Altogether, this study reveals that JSRV utilizes a pH-dependent, dynamin-associated endocytosis pathway for entry that differs from the classical pH-dependent entry pathway of vesicular stomatitis virus.