Possible role of IL‐2 deficiency for hypogammaglobulinaemia in patients with common variable immunodeficiency
Possible role of IL‐2 deficiency for hypogammaglobulinaemia in patients with common variable immunodeficiency
复制标题
IL-2 缺乏对常见变异型免疫缺陷患者低丙种球蛋白血症的可能作用
DOI：
10.1111/j.1365-2249.1992.tb06933.x
复制
发表时间：
1992
影响因子：
4.6
通讯作者：
Hans
中科院分区：
文献类型：
--
作者：
Rump Ja;A. Jahreis;M. Schlesier;R. Dräger;I. Melchers;Hans
Common variable immunodeficiency (CVID) patients are unable to produce specific immunoglobulins after antigen contact in vivo. The aim of this study was to investigate whether in some cases of CVID a decreased de novo synthesis of IL‐2 might be the cause of immunodeficiency and whether this deficiency can be corrected by IL‐2 supplementation in vitro. Mononuclear cells from 17 CVID patients and from 10 healthy controls were cultured with monoclonal anti‐CD3 antibody OKT3, pokeweed mitogen (PWM) or tetanus toxoid (TT) to stimulate IL‐2 synthesis. In parallel, in vitro IgG and IgM synthesis was stimulated with Staphylococcus aureus Cowan I (SAC), PWM or TT in the presence or absence of IL‐2. While lymphocytes of 11 out of 17 patients produced low to normal amounts of IL‐2 upon stimulation with anti‐CD3, only three patients showed low IL‐2 production in response to PWM and five in response to TT. Regarding immunoglobulin synthesis in vitro, five patients completely failed to produce IgM or IgG upon stimulation with PWM, SAC or TT irrespective of the addition of IL‐2. By contrast, four patients did not show any defect in vitro and synthesized normal amounts of IgM and IgG with any of the three stimuli. Finally, eight patients could be reconstituted for PWM‐, SAC‐ and TT‐induced IgM and/or IgG synthesis in vitro, by adding IL‐2 to the culture system. This enhancing effect of IL‐2 could be blocked by adding anti‐IL‐2 receptor antibodies to the cultures. Our findings indicate that a defective IL‐2 synthesis after antigen stimulation may be one reason for the impaired immunoglobulin production in some cases of CVID.
