Genetic diversity in muscadine and American bunch grapes based on randomly amplified polymorphic DNA (RAPD) analysis
Genetic diversity in muscadine and American bunch grapes based on randomly amplified polymorphic DNA (RAPD) analysis
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DOI：
10.21273/jashs.121.6.1020
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发表时间：
1996-11-01
影响因子：
1.9
通讯作者：
Lamikanra, O
中科院分区：
文献类型：
--
作者：
Qu, XP;Lu, J;Lamikanra, O
美国种植了两种形态不同的葡萄，分别属于葡萄属的真葡萄亚属和麝香葡萄亚属。前者通常被称为串葡萄，而后者通常被称为圆叶葡萄。使用 RAPD 标记研究了这些葡萄的遗传多样性。 RAPD 分析使用了 16 个葡萄品种，其亲本包括 V. rotundifolia Michx.、V. vinifera L. 和几个美洲葡萄品种。 19个随机引物共产生156个RAPD标记，其中90%以上在圆叶葡萄和串葡萄中具有多态性。每个亚属内的多态性较低。根据带共享和聚类分析估计这两个亚属之间的关系。该串与圆叶葡萄品种之间的平均遗传距离为0.45。基于 DNA 分析的结果与另一项研究获得的同工酶数据一致，该数据表明圆叶葡萄与美国串葡萄和欧洲葡萄几乎没有共同的等位基因。
Two morphologically distinct types of grapes belonging to the subgenera Euvitis and Muscadinia in the genus Vitis are cultivated in the United States. The former is commonly called bunch grapes while the latter is usually called muscadine. Genetic diversity among these grapes was investigated using RAPD markers. Sixteen grape cultivars, with parentage including V. rotundifolia Michx., V. vinifera L., and several American Vitis species, were used for the RAPD analysis. A total of 156 RAPD markers was produced from 19 random primers, over 90% of which was polymorphic among the muscadine and the bunch grapes. Polymorphisms were lower within each subgenus. Relationships between these two subgenera were estimated based on band-sharing and cluster analysis. The average genetic distance between the bunch and the muscadine grape cultivars was 0.45. The results based on DNA analysis agree with isozyme data obtained from a separate study, which demonstrated that muscadine grapes share very few common alleles with American bunch grapes and European grapes.