Bacillus selenatarsenatis sp. nov., a selenate- and arsenate-reducing bacterium isolated from the effluent drain of a glass-manufacturing plant.
Bacillus selenatarsenatis sp. nov., a selenate- and arsenate-reducing bacterium isolated from the effluent drain of a glass-manufacturing plant.
复制标题
DOI：
10.1099/ijs.0.64667-0
复制
发表时间：
2007-05
影响因子：
2.8
通讯作者：
Shigeki Yamamura;M. Yamashita;N. Fujimoto;M. Kuroda;Masami Kashiwa;K. Sei;M. Fujita;M. Ike
中科院分区：
文献类型：
--
作者：
Shigeki Yamamura;M. Yamashita;N. Fujimoto;M. Kuroda;Masami Kashiwa;K. Sei;M. Fujita;M. Ike
从日本一家玻璃制造厂的废水中分离到一株兼性厌氧的硒酸盐和砷酸盐还原菌，命名为SF-1（T）。该菌染色呈革兰氏阳性，是一种能动的芽孢杆菌，能够以硒酸盐、砷酸盐和硝酸盐作为末端电子受体进行呼吸。该菌株的主要细胞脂肪酸为异C（15：0）、异C（17：1）ω 10 c和C（16：1）ω 7 c醇。基因组DNA的G+C含量为42.8mol%。虽然最接近的系统发育邻居是芽孢杆菌jeotelicum JCM 10885（T），其16 S rRNA基因序列相似性为99.6%，但DNA-DNA杂交研究显示这些菌株之间的相关性仅为14%，这一水平明显低于划分不同物种的推荐值。这与表型差异（电子受体利用、NaCl耐受性）一起表明菌株SF-1（T）代表芽孢杆菌属的一个新物种，建议将其命名为硒砷芽孢杆菌新种。模式菌株为SF-1（T）（=JCM 14380（T）=DSM 18680（T））。
A facultatively anaerobic, selenate- and arsenate-reducing bacterium, designated strain SF-1(T), was isolated from a selenium-contaminated sediment obtained from an effluent drain of a glass-manufacturing plant in Japan. The bacterium stained Gram-positive and was a motile, spore-forming rod capable of respiring with selenate, arsenate and nitrate as terminal electron acceptors. The major cellular fatty acids of the strain were iso-C(15 : 0), iso-C(17 : 1)omega10c and C(16 : 1)omega7c alcohol. The G+C content of the genomic DNA was 42.8 mol%. Though the nearest phylogenetic neighbour was Bacillus jeotgali JCM 10885(T), with a 16S rRNA gene sequence similarity of 99.6 %, DNA-DNA hybridization studies showed only 14 % relatedness between these strains, a level that is clearly below the value recommended to delimit different species. This, together with the phenotypic differences (utilization of electron acceptors, NaCl tolerance), suggests that strain SF-1(T) represents a novel species of the genus Bacillus, for which the name Bacillus selenatarsenatis sp. nov. is proposed. The type strain is SF-1(T) (=JCM 14380(T)=DSM 18680(T)).