Characterization of the invasion of porcine endothelial cells by Streptococcus suis serotype 2.
Characterization of the invasion of porcine endothelial cells by Streptococcus suis serotype 2.
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发表时间：
2007-04
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通讯作者：
G. Vanier;M. Segura;M. Gottschalk
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作者：
G. Vanier;M. Segura;M. Gottschalk
猪链球菌2型是引起猪脑膜炎的重要病原菌。在以前的研究中，我们证明了S。猪血清2型粘附并侵袭形成血脑屏障的永生化猪脑微血管内皮细胞（PBMEC）。目前工作的目的是进一步表征S. SUIS侵入猪内皮细胞。几个S.猪群菌株与PBMEC相互作用与其地理来源、毒力、宿主来源或猪溶血素产生无关。表征研究表明，蛋白质粘附素/侵袭素、细胞壁组分、脂磷壁酸和血清组分（包括纤连蛋白）参与了S.猪和PBMEC。除PBMEC外，S. suis能够粘附并侵袭2个猪主动脉内皮细胞系和原代PBMEC。
Streptococcus suis serotype 2 is an important swine pathogen associated mainly with meningitis. In a previous study, we demonstrated the ability of S. suis serotype 2 to adhere to and invade immortalized porcine brain microvascular endothelial cells (PBMECs) forming the blood-brain barrier. The aim of the current work was to further characterize the mechanism(s) by which S. suis invades porcine endothelial cells. The ability of several S. suis strains to interact with PBMECs was not found to correlate with their geographic origin, virulence, host of origin, or suilysin production. Characterization studies demonstrated that proteinaceous adhesins/invasins, cell wall components, lipoteichoic acid, and serum components (including fibronectin) were involved in interactions between S. suis and PBMECs. In addition to PBMECs, S. suis was able to adhere to and invade 2 porcine aortic endothelial cell lines and primary PBMECs.