Assessment of locomotion in chlorine exposed mice by computer vision and neural networks
Assessment of locomotion in chlorine exposed mice by computer vision and neural networks
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DOI：
10.1152/japplphysiol.01023.2011
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发表时间：
2012-03-01
影响因子：
3.3
通讯作者：
Matalon, Sadis
中科院分区：
文献类型：
--
作者：
Filippidis, Aristotelis S.;Zarogiannis, Sotirios G.;Matalon, Sadis
[2]Filippidis as，Zarogiannis SG，Randich A，Ness TJ，Matalon S.用计算机视觉和神经网络评估氯气暴露小鼠的运动能力。应用生理学杂志112：1064-1072,2012。2011年12月29日首次出版；DOI：10.1152/japplPhysiol.01023.2011。-对动物暴露在有害或痛苦刺激下的运动进行评估，可以为了解伤害的潜在机制和各种治疗的有效性提供重要的见解。我们开发了一种新的方法，利用计算机视觉和神经网络算法来跟踪老鼠在二维的运动。通过使用该系统，我们证明了暴露在氯(Cl2-)气体中的小鼠在暴露后9小时内表现出运动能力受损和不动能力增加。暴露后给予丁丙诺啡，一种常见的止痛剂，增加了氯-2暴露的小鼠的活动和减少不动时间，但不是空气暴露，最可能是通过减少氯-2引起的疼痛。这种方法可以适用于评估暴露在各种化学和行为有害刺激下的各种治疗的有效性。
Filippidis AS, Zarogiannis SG, Randich A, Ness TJ, Matalon S. Assessment of locomotion in chlorine exposed mice by computer vision and neural networks. J Appl Physiol 112: 1064-1072, 2012. First published December 29, 2011; doi: 10.1152/japplphysiol.01023.2011.-Assessment of locomotion following exposure of animals to noxious or painful stimuli can offer significant insights into underlying mechanisms of injury and the effectiveness of various treatments. We developed a novel method to track the movement of mice in two dimensions using computer vision and neural network algorithms. By using this system we demonstrated that mice exposed to chlorine (Cl-2) gas developed impaired locomotion and increased immobility for up to 9 h postexposure. Post-exposure administration of buprenorphine, a common analgesic agent, increased locomotion and decreased immobility times in Cl-2- but not air-exposed mice, most likely by decreasing Cl-2-induced pain. This method can be adapted to assess the effectiveness of various therapies following exposure to a variety of chemical and behavioral noxious stimuli.