Immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections.
Immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections.
复制标题
组织切片上β-半乳糖苷酶或绿色荧光蛋白的免疫组织化学检测。
DOI：
10.1007/978-1-59745-549-7_2
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Sander,Maike
中科院分区：
文献类型：
--
作者：
Seymour,PhilipA;Sander,Maike
With the recent advances in mouse genetics, it is now possible to mark specific cell types genetically in vivo and to study the fate of cells during development and adulthood. Cells are labeled and followed in vivo through the stable expression of reporter genes in particular cell types. The two most commonly used reporter genes areLacZ, which encodes the enzyme β-galactosidase (β-gal), and green fluorescent protein (GFP). β-Gal expression can be detected enzymatically, using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as a substrate, and GFP can be directly visualized by fluorescence microscopy. However, with single detection of β-gal or GFP, it is often impossible to determine whether expression of the reporter protein is restricted to a particular cell type. To ascertain the identity of individual cells within a multicellular tissue, β-gal or GFP proteins must be visualized in conjunction with additional cellular markers. For such experiments, specific antibodies raised against β-gal or GFP can be used in coimmunofluorescence analyses. Such double-staining analyses on tissue sections are a powerful tool to study transgene expression or to trace cells in multicellular tissues.
微波柠檬酸盐抗原修复在诊断免疫组织化学中的应用。
影响因子：
7.5
作者：
R. Brown;R. Chirala
通讯作者：
R. Chirala