[Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes].
[Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes].
复制标题
多形核白细胞表面Mac-1表达增加导致肺血管通透性增加
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
S. Fujimura
中科院分区：
文献类型：
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作者：
T. Tanita;C. Song;S. Ueda;Y. Hoshikawa;S. Maeda;M. Noda;T. Tabata;S. Suzuki;S. Ono;S. Fujimura
我们研究了人多形核白细胞(PMN)表面黏附分子的表达。用流式细胞仪检测机械刺激的效果，用重量法评估肺内中性粒细胞聚集所致的肺血管损伤。通过测定髓过氧化物酶的含量来研究PMN在肺中的蓄积。在玻璃容器中轻轻搅拌刺激10个S的中性粒细胞，机械刺激的中性粒细胞表面表达Mac-1(CD11b/CD18)。未受刺激的中性粒细胞暴露于大鼠离体肺，滤过系数与基线条件下无明显变化。然而，当机械刺激的中性粒细胞暴露在离体大鼠肺中时，过滤系数约为基线时的5倍。当用抗CD18抗体处理机械刺激的PMN时，滤过率的增加完全被阻断。髓过氧化物酶检测显示，只有在刺激的PMN加入后，PMN才能附着在离体肺上。我们的结论是，当机械刺激增加PMN的黏附性时，这些细胞会黏附在肺血管上，并增加肺血管的通透性。
We studied the expression of adhesion molecules on the surface of human polymorphonuclear leukocytes (PMNs). The effects of mechanical stimulation were measured with a flow cytometer and pulmonary vascular injury due to accumulation of PMNs in the lungs was assessed by a gravimetric method. The accumulation of PMNs in the lungs was studied by measuring the amount of myeloperoxidase. PMNs were stimulated by gentle agitation in a glass container for 10 s. Mac-1 (CD11b/CD18) was upregulated on the surface of PMNs that were mechanically stimulated. When unstimulated PMNs were exposed to isolated rat lungs, the filtration coefficient did not change from that under baseline conditions. However, when mechanically stimulated PMNs were exposed to isolated rat lungs, the filtration coefficient was about 5 times higher than that measured at baseline. When mechanically stimulated PMNs treated with anti-CD18 antibody were used, the increase in the filtration coefficient was completely blocked. The assay of myeloperoxidase revealed that PMNs stuck to isolated rat lungs only after stimulated PMNs were added. We conclude that when the adhesiveness of PMNs is increased by mechanical stimulation, these cells adhere to pulmonary vessels and increase pulmonary vascular permeability.
影响因子：
56.9
作者：
KISHIMOTO, TK;JUTILA, MA;BUTCHER, EC
通讯作者：
BUTCHER, EC