Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)
Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)
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DOI：
10.1007/s001220051043
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发表时间：
1999-01-01
影响因子：
5.4
通讯作者：
Ranalli, P
中科院分区：
文献类型：
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作者：
Mandolino, G;Carboni, A;Ranalli, P
在大麻（Cannabis sativa L.）的14个雌雄异株品种和材料中，发现了一个由随机十聚体序列引物产生的400 bp RAPD标记与雄性表型相关。引物OPA8产生一组条带，其中大部分条带在所有被测植株中都存在多态性，其中1条条带命名为OPA8(400)，在所有雄性植株中都存在，而在雌性植株中不存在。对167株不同基因型的植物进行了性别表型与OPA8（400）标记关联的筛选，结果显示，在表型为雌性的植物中，只有3例存在400-bp条带；相反，在雄性植物中，条带从未缺失，而在雌雄同株植物中，条带从未存在。尽管存在这种性别特异性关联，但通过Southern blotting和克隆的RAPD条带杂交发现，与OPA8（400）对应的序列在雄蕊和无皮植物中都存在。对RAPD标记进行测序，构建特异性引物。这些引物在用于RAPD分析的相同基因型上产生了长度为390 bp的SCAR标记，并且具有雄性特异性。该SCAR适用于大麻雌雄异株和雌雄同株育种过程中对雄性植物进行精确、早期和快速的鉴定。
a 400-bp RAPD marker generated by a primer of random decamer sequence has been Found associated with the male sex phenotype in 14 dioecious cultivars and accessions of hemp (Cannabis sativa L.). The primer OPA8 generates a set of bands, most of which polymorphic among all the individual plants tested, and 1 of which, named OPA8(400), present in all male plants and absent in female plants. A screening of 167 plants belonging to different genotypes for the association of the OPA8(400) marker with the sex phenotype revealed that only in 3 cases was the 400-bp band was present in plants phenotypically female; on the contrary, in male plants the band was never missing, while in monoecious plants it was never present. Despite this sex-specific association, the sequences corresponding to OPA8(400) were present in both staminate and carpellate plants, as revealed by Southern blotting and hybridization with the cloned RAPD band. The RAPD marker was sequenced, and specific primers were constructed. These primers generated, on the same genotypes used for RAPD analysis, a SCAR marker 390 bp in length and male-specific. This SCAR is suitable for a precise, early and rapid identification of male plants during breeding programs of dioecious and monoecious hemp.