Encapsulation of grape seed extract in polylactide microcapsules for sustained bioactivity and time-dependent release in dental material applications.
Encapsulation of grape seed extract in polylactide microcapsules for sustained bioactivity and time-dependent release in dental material applications.
复制标题
将葡萄籽提取物封装在聚丙交酯微胶囊中，以在牙科材料应用中实现持续的生物活性和时间依赖性释放。
DOI：
10.1016/j.dental.2017.03.009
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
White,ScottR
中科院分区：
文献类型：
--
作者：
Yourdkhani,Mostafa;Leme-Kraus,ArieneArcas;Aydin,Berdan;Bedran-Russo,AnaKarina;White,ScottR
ObjectiveTo sustain the bioactivity of proanthocyanidins-rich plant-derived extracts via encapsulation within biodegradable polymer microcapsules.MethodsPolylactide microcapsules containing grape seed extract (GSE) were manufactured using a combination of double emulsion and solvent evaporation techniques. Microcapsule morphology, size distribution, and cross-section were examined via scanning electron microscopy. UV–vis measurements were carried out to evaluate the core loading and encapsulation efficiency of microcapsules. The bioactivity of extracts was evaluated after extraction from capsules via solvent partitioning one week or one year post-encapsulation process. Fifteen human molars were cut into 7 mm × 1.7 mm × 0.5 mm thick mid-coronal dentin beams, demineralized, and treated with either encapsulated GSE, pristine GSE, or left untreated. The elastic modulus of dentin specimens was measured based on three-point bending experiments as an indirect assessment of the bioactivity of grape seed extracts. The effects of the encapsulation process and storage time on the bioactivity of extracts were analyzed.ResultsPolynuclear microcapsules with average diameter of 1.38 μm and core loading of up to 38 wt% were successfully manufactured. There were no statistically significant differences in the mean fold increase of elastic modulus values among the samples treated with encapsulated or pristine GSE (p = 0.333), or the storage time (one week versus one year storage at room temperature, p = 0.967).SignificancePolynuclear microcapsules containing proanthocyanidins-rich plant-derived extracts were prepared. The bioactivity of extracts was preserved after microencapsulation.
