Occurrence of 3-Methylaspartate Ammonia-lyase in Facultative Anaerobes and Their Application to Synthesis of 3-Substituted (S)-Aspartic Acids.
Occurrence of 3-Methylaspartate Ammonia-lyase in Facultative Anaerobes and Their Application to Synthesis of 3-Substituted (S)-Aspartic Acids.
复制标题
兼性厌氧菌中 3-甲基天冬氨酸解氨酶的出现及其在 3-取代 (S)-天冬氨酸合成中的应用。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Yasuo Kato
中科院分区：
文献类型：
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作者：
Y. Asano;Yasuo Kato
从土壤中分离到4株产3-甲基天冬氨酸解氨酶（MAL）的兼性厌氧菌。它们被鉴定为柠檬酸杆菌属0504号、变形杆菌属0601号、大肠杆菌1002号和肠杆菌属1202号。在厌氧培养或静态培养条件下，（S）-谷氨酸或（2S，3S）-3-甲基天冬氨酸均可诱导该酶的活性。利用分离物的无细胞提取物，分别以甲磺酸、乙基富马酸和氯富马酸为原料，合成了光学纯的3-取代（S）-天冬氨酸衍生物，如（2S，3S）-3-甲基天冬氨酸、（2S，3S）-3-乙基天冬氨酸和（2 R，3S）-3-氯天冬氨酸。
Four facultative anaerobes were isolated from soil as producers of 3-methylaspartate ammonia-lyase (MAL). They were identified as Citrobacter sp. No. 0504, Proteus sp. No. 0601, Escherichia coli No. 1002, and Enterobacter sp. No. 1202. The enzyme activities were induced when the strains were cultivated anaerobically or statically in the presence of (S)-glutamic acid or (2S, 3S)-3-methylaspartic acid. Using cell-free extracts of the isolates, several 3-substituted (S)-aspartic acid derivatives such as (2S,3S)-3-methylaspartic acid, (2S,3S)-3-ethylaspartic acid, and (2R,3S)-3-chloroaspartic acid were synthesized in optically pure form from mesaconic acid, ethylfumaric acid, and chlorofumaric acid, respectively.