Microsatellite loci for Myrmica kotokui and their application in some congeneric ant species distributed in northern Japan

Microsatellite loci for Myrmica kotokui and their application in some congeneric ant species distributed in northern Japan
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DOI:
10.1111/j.1471-8286.2004.00849.x
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发表时间:
2005-03-01
期刊:
MOLECULAR ECOLOGY NOTES
影响因子:
--
通讯作者:
Higashi, S
Higashi, S
中科院分区:
其他
文献类型:
--
作者:
Azuma, N;Takahashi, J;Higashi, S

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我们从 RAPD(多态性 DNA 快速分析)片段中开发了 Myrmica kotokui 的 7 个多态性微卫星位点。这些基因座显示出 2 至 6 个等位基因,预期和观察到的杂合度分别为 0.13-0.73 和 0.14-0.78。这些基因座可转移至来自日本北部的同属物种,如红杨梅(Myrmica rubra)、木黄杨梅(Myrmica uteola)和缨桃杨(Myrmica taediosa)。这些基因座将允许在群体水平和群体水平上分析 Myrmica 物种的遗传结构。
We developed seven polymorphic microsatellite loci for Myrmica kotokui from RAPD (rapid analysis of polymorphic DNA) fragments. These loci showed two to six alleles with expected and observed heterozygosity of 0.13-0.73 and 0.14-0.78, respectively. These loci are transferable to the congeneric species as Myrmica rubra, Myrmica luteola and Myrmica taediosa from northern Japan. These loci will allow analyses of genetic structure of Myrmica species at both the colony level and population levels.