Toxicity Assessment of Nine Types of Decoction Pieces from the Daughter Root of Aconitum carmichaeli (Fuzi) Based on the Chemical Analysis of their Diester Diterpenoid Alkaloids
Toxicity Assessment of Nine Types of Decoction Pieces from the Daughter Root of Aconitum carmichaeli (Fuzi) Based on the Chemical Analysis of their Diester Diterpenoid Alkaloids
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DOI：
10.1055/s-0029-1240688
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发表时间：
2010-05-01
期刊：
影响因子：
2.7
通讯作者：
Zhao, Zhongzhen
中科院分区：
文献类型：
--
作者：
Lu, Guanghua;Dong, Zhengqi;Zhao, Zhongzhen
附子（剧毒植物Aconitum carmichaeli Debx的子根，FZ）的各种炮制饮片（按照中药手册规格炮制的草药材料；中文音译“饮片”）在传统医学中广泛用于治疗各种疾病，但其毒性尚不清楚。因此，制备了九种FZ饮片，包括一种生片和八种炮制品，每批7至10批，以评估其毒性。采用新开发的 HPLC-DAD 和 LC-MS 技术，对 84 个 FZ 样品中的高毒性二酯二萜生物碱（即乌头碱、新乌头碱和次乌头碱）的含量进行了定量。将处理后的 FZ 与原始根切片进行比较表明，处理后的 FZ 中每种分析物的含量均大幅下降。 8种加工品中的3种有毒化合物含量之和仅为生片中该值的3.91-34.80%。这意味着加工后的 FZ 的毒性显着降低。 8种炮制品中的有毒成分含量差异较大，往往相差10次方，临床用药时应谨慎设定用药剂量，以免发生中毒事件。
Various processed types of Fuzi (the daughter roots of the highly toxic plant Aconitum carmichaeli Debx, FZ) decoction pieces (the herbal materials processed according to the specifications of Chinese medicine manuals; "Yinpian" in Chinese transliteration) are widely used in traditional medicine to treat various diseases, but their toxicities are not known. Nine types of FZ decoction pieces, including one raw slice and eight processed forms, were therefore prepared, each in 7 to 10 batches, to assess for their toxicity. Altogether 84 FZ samples were quantified on the amount of highly toxic diester diterpenoid alkaloids, i.e., aconitine, mesaconitine and hypaconitine by a newly developed HPLC method with HPLC-DAD and LC-MS techniques. The comparison of the processed FZ to raw slices of the root showed that the amount of each analyte in the processed FZ was drastically decreased. The sum of the three toxic compounds in the 8 types of processed FZ was only 3.91-34.80% of this value in the FZ raw slice. This implies that the toxicity of processed FZ was decreased significantly. The amounts of toxic components in the 8 types of processed FZ varied significantly, often by a power of ten, indicating that the dosage of these herbs, when prescribed for clinical uses, should be cautiously set in order to avoid poisoning incidents.