Modulation of Vocal Fold Scar Fibroblasts By Adipose-Derived Stem/Stromal Cells
Modulation of Vocal Fold Scar Fibroblasts By Adipose-Derived Stem/Stromal Cells
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脂肪干细胞/基质细胞对声带褶皱疤痕成纤维细胞的调节作用
DOI：
10.1002/lary.20753
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发表时间：
2010-02-01
期刊：
影响因子：
2.6
通讯作者：
Zeitels, Steven M.
中科院分区：
文献类型：
--
作者：
Kumai, Yoshihiko;Kobler, James B.;Zeitels, Steven M.
目的/假设：通过分泌型肝细胞生长因子(HGF)对瘢痕成纤维细胞的影响，探讨脂肪来源的干细胞/基质细胞(ASCs)是否具有治疗瘢痕固有层浅层瘢痕的潜能。研究设计：采用共培养体系进行体外研究。方法：从2周前电灼的雪貂声带分离出瘢痕成纤维细胞(SFS)。从雪貂腹部皮下脂肪中分离出ASCs。在共培养实验中，将两种类型的细胞在Transwell平板中组合6天，然后在去除上腔后进行1或3天的单一培养。然后对底部小室的细胞和培养液进行分析。我们检测了：1)用酶联免疫吸附试验检测透明质酸(RA)、胶原和HGF的产生，2)α-平滑肌肌动蛋白(α-SMA)的表达，3)细胞增殖，4)SFS(2，3和4)的凋亡。其他实验检测HGF对SFS的影响以及HGF中和在共培养体系中的作用。结果：共培养使SF胶原产量显著减少(P
Objectives/Hypothesis: To explore whether adipose-derived stem/stromal cells (ASCs) have therapeutic potential for treating scarred superficial lamina propria through the effects of secreted hepatocyte growth factor (HGF) on scar fibroblasts.Study Design: In vitro study using coculture system.Methods: Scar fibroblasts (SFs) were isolated from ferret vocal folds electrocauterized 2 weeks previously. ASCs were isolated from ferret lipoaspirated subcutaneous abdominal fat. For coculture experiments, the two cell types were combined in Transwell plates for 6 days, followed by 1 or 3 days of monoculture after removing the upper chamber. Assays were then performed on cells and media from the bottom chamber. We measured: 1) the production of hyaluronic acid (RA), collagen and HGF via enzyme-linked immunosorbent assays, 2) the expression of alpha-smooth muscle actin (alpha-SMA), 3) cell proliferation, and 4) apoptosis of SFs (2, 3, and 4 via flow cytometry). Other experiments examined the effects of HGF on SFs and the effects of HGF neutralization in the coculture system.Results: Coculture led to significant decreases in SF collagen production (P