Fundamental Limits on Measuring the Rotational Constraint of Single Molecules Using Fluorescence Microscopy
Fundamental Limits on Measuring the Rotational Constraint of Single Molecules Using Fluorescence Microscopy
复制标题
使用荧光显微镜测量单分子旋转约束的基本限制
DOI：
10.1103/physrevlett.122.198301
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发表时间：
2019
影响因子：
8.6
通讯作者：
Lew, Matthew D.
中科院分区：
文献类型：
--
作者：
Zhang, Oumeng;Lew, Matthew D.
光学荧光成像能够测量单个分子的平移和旋转动力学。然而，不可避免的测量噪声将导致旋转动力学的不准确估计，导致分子看起来比实际上更受旋转约束。我们报告了一个数学框架来计算的基本限制的准确性，在测量的偶极发射器的旋转流动性。通过将我们的框架应用于平面和三维方法，我们提供了一种根据实验条件选择最佳取向测量技术的方法。
Optical fluorescence imaging is capable of measuring both the translational and rotational dynamics of single molecules. However, unavoidable measurement noise will result in inaccurate estimates of rotational dynamics, causing a molecule to appear to be more rotationally constrained than it actually is. We report a mathematical framework to compute the fundamental limit of accuracy in measuring the rotational mobility of dipolelike emitters. By applying our framework to both in-plane and three-dimensional methods, we provide a means to choose the optimal orientation-measurement technique based on experimental conditions.