ESCHERICHIA-COLI RECA PROTEIN POSSESSES A STRAND SEPARATING ACTIVITY ON SHORT DUPLEX DNAS
ESCHERICHIA-COLI RECA PROTEIN POSSESSES A STRAND SEPARATING ACTIVITY ON SHORT DUPLEX DNAS
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DOI：
10.1002/j.1460-2075.1985.tb04039.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
MAGNI, G
中科院分区：
文献类型：
--
作者：
BIANCHI, M;RIBOLI, B;MAGNI, G
RecA 蛋白被发现可催化 DNA 底物链的解离，该底物由退火至环状单链 M13mp DNA 的 20 核苷酸引物组成。链分离反应需要 ATP 水解并且需要解开双链 DNA 区域侧翼的单链 DNA。 RecA 催化的链分离仅对非常短的双链体（不超过 30 bp）有效，并且不受单链 DNA 结合蛋白的刺激。这些结果与recA蛋白破坏单链DNA二级结构区域并将大的非同源性掺入异源双链DNA的能力一致。
RecA protein was found to catalyze the dissociation of the strands of a DNA substrate consisting of a 20-nucleotide primer annealed to circular single-stranded M13mp DNA. The strand separation reaction requires ATP hydrolysis and the presence of single-stranded DNA flanking the duplex DNA region to be unwound. RecA-catalyzed strand separation is effective only for very short duplexes, not exceeding 30 bp, and is not stimulated by single-stranded DNA-binding protein. These results are consistent with the ability of recA protein to disrupt regions of secondary structure in single-stranded DNA and to incorporate large non-homologies into heteroduplex DNA.