Tetraploid production through zygotic chromosome doubling in Populus
Tetraploid production through zygotic chromosome doubling in Populus
复制标题
通过杨属合子染色体加倍产生四倍体
DOI：
10.14214/sf.932
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发表时间：
2013-01-01
期刊：
影响因子：
1.8
通讯作者：
Kang, Xiangyang
中科院分区：
文献类型：
--
作者：
Wang, Jun;Shi, Le;Kang, Xiangyang
培育三倍体杨树最直接的途径是异源四倍体与二倍体杂交。然而，由于缺乏异源四倍体杨树，限制了该方法的应用。本研究采用秋水仙素和高温诱导合子型染色体加倍，获得了（小叶杨×浙银3#杨×北京杨）异源四倍体。通过秋水仙素处理和高温处理分别筛选出6个和25个四倍体个体，表明秋水仙素和高温处理均能有效地诱导杨树合子染色体加倍产生四倍体。子房中种毛的发育特征与合子的发育存在时间上的相关性，这一点成功地指导了秋水仙素和高温处理。在毛发发育的某些阶段，四倍体产生的效率显着较高。而秋水仙素浓度、温度、高温处理时间等因素对产量的影响不显著。四倍体和二倍体植株叶片气孔的大小和频率存在显著差异，表明这一性状可以通过材料基因组的增加而改变。本研究获得的异源四倍体具有不同的基因型，为进一步的三倍体育种提供了重要的亲本种质。
The most direct approach in breeding triploid Populus is crossing allotetraploids with diploids. However, the lack of allotetraploid Populus restricts application of this approach. In this investigation, zygotic chromosome doubling was induced with colchicine and high temperatures to produce ((Populus pseudo-simonii × P. nigra ‘Zheyin3#’) × (P. × beijingensis)) allotetraploids. We screened 6 and 25 tetraploid individual offspring from the colchicine and high-temperature treatments respectively, indicating that both colchicine and high temperature are effective for tetraploid production by zygotic chromosome doubling of Populus. Developmental characteristics of seed hairs in the ovaries were temporally associated with zygotic development, which was used to successfully guide the colchicine and high-temperature treatments. During certain stages of hair development, the efficiency of tetraploid production was significantly high. However, efficiency of production was not significantly influenced by other factors, i.e. colchicine concentration, temperature or duration of high-temperature treatment. Size and frequency of leaf stomata between tetraploid and diploid plants were significantly different, suggesting that this character can be altered via genomic increase in material. The allotetraploids produced in this investigation, having different genotypes, provide important parental germplasms for further triploid breeding.