Capturing the mechanics of clathrin-mediated endocytosis
Capturing the mechanics of clathrin-mediated endocytosis
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DOI：
10.1016/j.sbi.2022.102427
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发表时间：
2022-07-21
影响因子：
6.8
通讯作者：
Smith，Corinne J.
中科院分区：
文献类型：
--
作者：
Smith，Sarah M.;Smith，Corinne J.
网状蛋白介导的内吞作用使分子能够选择性地吸收到细胞中，以响应不断变化的细胞需求。它通过在质膜周围组装涂层成分来确定囊泡的含量，并促进膜的弯曲形成笼状蛋白涂层的运输囊泡。在这篇综述中，我们讨论了最近的冷冻电子显微镜结构，这些结构捕捉到了笼蛋白包裹的囊泡生命周期中的一系列事件。单颗粒分析和断层扫描方法都揭示了笼状蛋白晶格结构本身的细节，AP2如何在包被的囊泡中与笼状蛋白结合，以及PIP2结合对于在膜上组装酵母适配器Sla2和Ent1的重要性。在细胞内，扁平格子中笼状蛋白的冷冻电子断层扫描和高速原子力显微镜研究为笼状蛋白在CCV形成过程中的形态如何适应提供了新的见解。因此，驱动胞苷介导的内吞作用的关键机械过程已经通过多种技术协同工作而被捕获。
Clathrin-mediated endocytosis enables selective uptake of molecules into cells in response to changing cellular needs. It occurs through assembly of coat components around the plasma membrane that determine vesicle contents and facilitate membrane bending to form a clathrin-coated transport vesicle. In this review we discuss recent cryo-electron microscopy structures that have captured a series of events in the life cycle of a clathrin-coated vesicle. Both single particle analysis and tomography approaches have revealed details of the clathrin lattice structure itself, how AP2 may interface with clathrin within a coated vesicle and the importance of PIP2 binding for assembly of the yeast adaptors Sla2 and Ent1 on the membrane. Within cells, cryo-electron tomography of clathrin in flat lattices and high-speed AFM studies provided new insights into how clathrin morphology can adapt during CCV formation. Thus, key mechanical processes driving clathrin-mediated endocytosis have been captured through multiple techniques working in partnership.