ANTIBACTERIAL ACTION OF CHITOSAN
ANTIBACTERIAL ACTION OF CHITOSAN
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DOI：
10.1080/08905439209549838
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发表时间：
1992-01-01
影响因子：
1.8
通讯作者：
KNORR, D
中科院分区：
文献类型：
--
作者：
SUDARSHAN, NR;HOOVER, DG;KNORR, D
研究了壳聚糖谷氨酸盐（CH）、壳聚糖乳酸盐（CL）和真菌菌丝体壳聚糖对革兰氏阴性菌和革兰氏阳性菌的抗菌作用。平板计数表明1小时内1 - 5个对数循环的灭活率。真菌壳聚糖的抑菌效果明显低于CH和CL。CH和CL的抗菌作用非常相似，并且显示出浓度依赖性，0.1 mg/mL比2.0和5.0 mg/mL更有效。当CH（或CL）和聚半乳糖醛酸盐加入到细胞悬浮液中，死亡被阻止，可能表明壳聚糖与聚半乳糖醛酸盐复合不能穿透细胞或破坏膜。通过将乳糖诱导的大肠杆菌暴露于壳聚糖来确定壳聚糖引起的细胞内组分的泄漏，β-半乳糖苷酶活性测定表明细胞透化在低壳聚糖浓度下发生得更广泛。显微镜检查表明，壳聚糖在pH 5.8时引起细胞凝集。使用亚致死应激细胞对高水平氯化钠的敏感性增加的标准，不能证明壳聚糖暴露的金黄色葡萄球菌MF-31的损伤。
The antibacterial action of chitosan hydroglutamate (CH), chitosan lactate (CL) and chitosan derived from fungal mycelia was examined against both gram-negative and gram-positive bacteria. Plate counts indicated inactivation rates of one- to five-log-cycles within one hour. Fungal chitosan had significantly less antibiotic effect than CH and CL. The antibacterial action of CH and CL was very similar and shown to be concentration dependent with 0.1 mg/mL more effective than 2.0 and 5.0 mg/mL. When CH (or CL) and polygalacturonate were added to cell suspensions, death was prevented, possibly indicating that chitosan complexed with polygalacturonate could not penetrate the cell or disrupt the membrane. Leakage of intracellular components caused by chitosan was determined by exposing lactose-induced Escherichia coli to chitosan with assay for beta-galactosidase activity indicating that cell permeabilization occurred more extensively at the low chitosan concentrations. Microscopic examination showed that chitosan caused cell agglutination at pH 5.8. Injury to chitosan-exposed Staphylococcus aureus MF-31 could not be demonstrated using the criterion that sublethally stressed cells have increased sensitivity to high levels of sodium chloride.