Cloning and sequencing of cDNA encoding the putative insect plasma membrane V-ATPase subunit A.
Cloning and sequencing of cDNA encoding the putative insect plasma membrane V-ATPase subunit A.
复制标题
编码假定的昆虫质膜 V-ATP 酶亚基 A 的 cDNA 的克隆和测序。
DOI：
10.1016/0014-5793(92)80177-i
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
Wieczorek,H
中科院分区：
文献类型：
--
作者：
Gräf,R;Novak,FJ;Harvey,WR;Wieczorek,H
首次对无脊椎动物v - atp酶a亚基的cDNA进行了测序。从烟角虫幼虫中肠制备cDNA文库。从cDNA序列中预测，昆虫亚基A由617个氨基酸组成，相对分子质量为68.162。预测的一级结构与已发表的真核亚基A蛋白（Bos,Daucus,SaccharomycesandNeurospora）相似；它最接近于牛的氨基酸序列，它有83%的序列同一性
For the first time a cDNA encoding subunit A of an invertebrate V-ATPase has been sequenced. The cDNA library was prepared from larval midgut of the tobacco hornworm.Manduca sexta, and screened with monoclonal antibodies to the midgut plasma membrane subunit A. From the cDNA sequence the insect subunit A is predicted to consist of 617 amino acids with a relative molecular mass of 68.162. The predicted primary structure is similar to that of the published eukaryotic subunit A proteins (Bos,Daucus,SaccharomycesandNeurospora); it most closely resembles the bovine amino acid sequences with which it has an 83% sequence identity