Characteristics of root boron nutrition confer high boron efficiency in Brassica napus cultivars
Characteristics of root boron nutrition confer high boron efficiency in Brassica napus cultivars
复制标题
根部硼营养特性赋予甘蓝型油菜品种高硼效率
DOI：
10.1007/s11104-013-1669-1
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发表时间：
2013-10-01
期刊：
影响因子：
4.9
通讯作者：
Xu, Fangsen
中科院分区：
文献类型：
--
作者：
Yang, Lu;Zhang, Quan;Xu, Fangsen
背景与目的甘蓝型油菜对硼（B）有较高的需求，但在缺B方面存在显著的基因型差异。为了阐明甘蓝型油菜品种对低B胁迫的不同敏感性与B吸收和转运特性之间的关系，探讨甘蓝型油菜B效率的调控。方法采用稳定同位素10 B示踪和嫁接试验，比较甘蓝型油菜高效和低效品种对B的吸收和转运，结果硼高效品种在B限制方面具有明显的优势。硼高效品种HZ表现出较低的B缺乏症状和较高的干生物量比硼低效品种LW和LB。硼高效品种HZ地上部和根系中的总B量和10 B浓度及积累量均高于硼低效品种。B低效LW向地上部运输的总B和10 B量均低于其他3个品种，而B低效LB根系中总B和10 B的含量和积累量均最低。以硼高效品种HZ的根系为砧木，无论是硼高效品种还是硼低效品种，嫁接植株均表现出硼高效的特征，表现出较轻的B缺乏症状、较高的干生物量和B积累量。相反，嫁接植物与B-低效LW用作股票是B-低效。与正常B胁迫相比，低B胁迫下BnBOR 1;1c、BnBOR 1;2a和BnNIP 5;1基因在根中的表达均上调。然而，有没有明显的差异，在低或正常B environment.ConclusionsThese结果表明，B-高效和B-低效品种之间的三个基因或其他四个BnBOR 1 s的表达，甘蓝型油菜的B效率主要是由根控制，这使得更多的吸收和积累的B在B-高效品种比B-低效品种在低B环境。然而，调控甘蓝型油菜B效率的分子机制仍有待确定。
Background and aimsBrassica napus has high boron (B) demand, but significant genotype differences exist with respect to B deficiency. The aim of this research was to elucidate the relationship between the different sensitivities of Brassica napus cultivars to low B stress and the characteristics of B uptake and transport to characterise the regulation of B efficiency in Brassica napus.MethodsB-efficient and B-inefficient Brassica napus cultivars were used to compare the uptake and transport of B using the stable isotope 10B tracer and grafting experiments, as well as expression of B transporters by RT-PCR.ResultsB-efficient cultivars have significant advantages with regard to B limitation. The B-efficient cultivar HZ showed less severe B deficiency symptoms and higher dry biomass than the B-inefficient cultivars LW and LB. Both the amount of total B and the 10B concentration and accumulation in the shoots and roots of B-efficient HZ were higher than those of B-inefficient cultivars. In B-inefficient LW, the amount of total B and the 10B that was transported into shoots was less than in the other three cultivars and the content and accumulation of total B and 10B in the roots of B-inefficient LB were the lowest among all of the cultivars. When the roots of B-efficient HZ were used as stocks, the grafted plants showed B-efficient characteristics, such as mild B deficiency symptoms, and higher dry biomass and B accumulation, regardless of whether they originated from B-efficient or B-inefficient cultivars. In contrast, the grafted plants with B-inefficient LW used as stocks were B-inefficient. The expressions of BnBOR1;1c, BnBOR1;2a and BnNIP5;1 were up-regulated in roots under low B stress compared with the normal B condition. However, there was no obvious difference in the expressions of the three genes or of four other BnBOR1s between B-efficient and B-inefficient cultivars in low or normal B environments.ConclusionsThese results indicate that the B efficiency of Brassica napus is controlled primarily by roots, which allow more uptake and accumulation of B in B-efficient cultivars than B-inefficient cultivars in a low B environment. However the molecular mechanism regulating B efficiency in Brassica napus remains to be determined.