Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells

Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells
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用于哺乳动物细胞中光控基因表达的笼状线性 DNA 的制备和基于亲和力的纯化

DOI：
10.1039/c3cc46607h
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发表时间：
2014
期刊：
Chem. Commun.
影响因子：
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通讯作者：
S. Watanabe and T. Furuta
S. Watanabe and T. Furuta
中科院分区：
--
文献类型：
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作者：
A. Teraoka;K. Murakoshi;K. Fukamauchi;A. Z. Suzuki;S. Watanabe and T. Furuta

文献摘要

相似文献

建立了一种简便实用的笼状DNA制备方法。该方法包括具有最小蛋白质表达序列的线性dsDNA与Bio-Bhc-diazo的笼化反应以及笼化DNA的亲和分离。证明了蛋白表达的有效抑制和光介导的恢复。
A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.