THE ENTRY OF JUNIN VIRUS INTO VERO CELLS
THE ENTRY OF JUNIN VIRUS INTO VERO CELLS
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DOI：
10.1007/bf01321064
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发表时间：
1994-01-01
影响因子：
2.7
通讯作者：
DAMONTE, EB
中科院分区：
文献类型：
--
作者：
CASTILLA, V;MERSICH, SE;DAMONTE, EB
研究了朱宁病毒(Junin Virus，JV)进入Vero细胞的机制，分析了亲水化合物和酸性pH对JV感染的影响。氯化铵、金刚烷胺、扑尔敏和普鲁卡因抑制JV的产生。氯化铵的作用是在感染早期发挥的。病毒内化受到抑制，未检测到病毒蛋白表达。当胞外培养液在低pH条件下缓冲时，氯化铵对JV感染的抑制作用被克服。此外，JV能在pH 5.5条件下诱导感染细胞融合，形成多核细胞。综上所述，这些结果表明JV通过内吞机制进入，需要低pH依赖的膜融合。
The entry mechanism of Junin virus (JV) into Vero cells was studied analyzing the effect oflysosomotropic compounds and acid pH on JV infection. Ammonium chloride, amantadine, chlorpheniramine and procaine inhibited JV production. The action of ammonium chloride was exerted at early times of infection. Virus internalization was inhibited and viral protein expression was not detected. When the extracellular medium was buffered at low pH, the ammonium chloride induced block on JV infection was overcome. Furthermore, JV was able to induce fusion of infected cells at pH 5.5 leading to polykaryoctye formation. Taken together, these results demonstrate that JV entry occurs through an endocytic mechanism requiring a low pH dependent membrane fusion.