RABBIT LIVER MICROSOMAL LIPID-PEROXIDATION - THE EFFECT OF LIPID ON THE RATE OF PEROXIDATION
RABBIT LIVER MICROSOMAL LIPID-PEROXIDATION - THE EFFECT OF LIPID ON THE RATE OF PEROXIDATION
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DOI：
10.1016/0005-2760(82)90077-7
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
AUST, SD
中科院分区：
文献类型：
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作者：
TIEN, M;AUST, SD
在螯合物ADP-Fe 3+存在下，大鼠和兔肝微粒体催化内源性脂质的NADPH-细胞色素P-450还原酶依赖性过氧化。虽然这两个物种的肝微粒体中含有相当水平的NADPH-细胞色素P-450还原酶和细胞色素P-450，但兔肝微粒体催化的脂质过氧化反应（通过丙二醛和脂质过氧化氢形成测定）速率仅为大鼠肝微粒体催化的约40%。用提取的微粒体脂质和纯化的大鼠和兔肝微粒体蛋白酶溶解的NADPH-细胞色素P-450还原酶制成的脂质体重建微粒体脂质过氧化反应。兔肝微粒体较低的脂质过氧化反应速率不能归因于还原酶的比活性。兔肝微粒体脂质对脂质过氧化反应的敏感性较低。这是由于兔肝微粒体脂质中多不饱和脂肪酸含量较低，而不是抗氧化剂的存在。微粒体脂质过氧化过程中损失的脂肪酸的GLC分析表明，脂肪酸不饱和度与脂质过氧化反应的速率相关。
Rat and rabbit liver microsomes catalyze an NADPH-cytochrome P-450 reductase-dependent peroxidation of endogenous lipid in the presence of the chelate ADP-Fe3+. Although liver microsomes from both species contain comparable levels of NADPH-cytochrome P-450 reductase and cytochrome P-450, the rate of lipid peroxidation (assayed by malondialdehyde and lipid hydroperoxide formation) catalyzed by rabbit liver microsomes is only about 40% of that catalyzed by rat liver microsomes. Microsomal lipid peroxidation was reconstituted with liposomes made from extracted microsomal lipid and purified protease-solubilized NADPH-cytochrome P-450 reductase from both rat and rabbit liver microsomes. The lower rates of lipid peroxidation catalyzed by rabbit liver microsomes could not be attributed to the specific activity of the reductase. Microsomal lipid from rabbit liver was much less susceptible to lipid peroxidation. This was due to the lower polyunsaturated fatty acid content rather than the presence of antioxidants in rabbit liver microsomal lipid. GLC analysis of fatty acids lost during microsomal lipid peroxidation revealed that the degree of fatty acid unsaturation correlated well with rates of lipid peroxidation.