Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts
Silver-nanoparticles increase bactericidal activity and radical oxygen responses against bacterial pathogens in human osteoclasts
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DOI：
10.1016/j.nano.2017.11.006
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发表时间：
2018-02-01
影响因子：
5.4
通讯作者：
Walch, Michael
中科院分区：
文献类型：
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作者：
Aurore, Valerie;Caldana, Fabienne;Walch, Michael
骨感染很难治疗，并可能导致严重的组织破坏。急性骨感染通常由金黄色葡萄球菌引起。属于单核细胞/巨噬细胞谱系的破骨细胞是骨感染的关键细胞。它们不具备杀死细菌的能力，可能成为细菌病原体的储存库。几个世纪以来，银因其杀菌活性而闻名。在这里，我们研究了纳米银颗粒在细菌感染的人破骨细胞中的杀菌作用。我们发现，纳米银本身无毒浓度的破骨细胞对细胞内耐甲氧西林，毒性金黄色葡萄球菌的杀菌活性增强。纳米银预处理的细胞中细菌存活率降低与对入侵病原体的活性氧反应增加相关。总体而言，这些结果表明，纳米银化合物应被视为细菌性骨和骨科植入物感染的有效治疗和预防选择。(c)2017作者爱思唯尔公司出版这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
Bone infections are difficult to treat and can lead to severe tissue destruction. Acute bone infections are usually caused by Staphylococcus aureus. Osteoclasts, which belong to the monocyte/macrophage lineage, are the key cells in bone infections. They are not well equipped for killing bacteria and may serve as a reservoir for bacterial pathogens. Silver has been known for centuries for its bactericidal activity. Here, we investigated the bactericidal effects of nano-silver particles in bacteria infected human osteoclasts. We found that nano-silver in per se nontoxic concentration enhanced the bactericidal activity in osteoclasts against intracellular Methicillin-resistant, virulent Staphylococcus aureus. The reduced bacterial survival in nano-silver pretreated cells correlated with increased reactive oxygen responses towards the invading pathogens. Overall, these results indicate that nano-silver compounds should be considered as an effective treatment and prevention option for bacterial bone and orthopedic implant infections. (c) 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).