Identification of a major QTL controlling the content of B-type starch granules in Aegilops.
Identification of a major QTL controlling the content of B-type starch granules in Aegilops.
复制标题
DOI：
10.1093/jxb/erq423
复制
发表时间：
2011-03
影响因子：
6.9
通讯作者：
Trafford K
中科院分区：
文献类型：
--
作者：
Howard T;Rejab NA;Griffiths S;Leigh F;Leverington-Waite M;Simmonds J;Uauy C;Trafford K
小麦族大多数物种胚乳中的淀粉具有独特的双峰颗粒形态，包括大的透镜状A型颗粒和较小的近球形B型颗粒。然而，一些野生小麦物种（山羊草属）已知缺乏B-颗粒。AE. peregrina和一个合成的四倍体山羊草具有相同的基因组组成（SU）被发现不同的B-颗粒数。人工合成的四倍体具有正常的A型和B型淀粉粒，而Ae. peregrina只有A颗粒，因为B颗粒未能启动。通过这两份材料的杂交，产生了一个B粒数分离的群体，并用于研究B粒发生的遗传基础。群体分离分析和QTL定位相结合，确定了一个位于4S染色体短臂上的主效QTL，占44.4%的表型变异。在具有不同基因组的多倍体山羊草中缺乏B-颗粒，这表明在小麦族的进化过程中，B-颗粒位点已经多次独立丢失。有人提出，B-颗粒基因座是容易沉默的多倍体化和模型的基础上，假设B-颗粒的启动是由一个单一的主基因座控制的单倍体基因组，以解释所观察到的数据。
Starch within the endosperm of most species of the Triticeae has a unique bimodal granule morphology comprising large lenticular A-type granules and smaller near-spherical B-type granules. However, a few wild wheat species (Aegilops) are known to lack B-granules. Ae. peregrina and a synthetic tetraploid Aegilops with the same genome composition (SU) were found to differ in B-granule number. The synthetic tetraploid had normal A- and B-type starch granules whilst Ae. peregrina had only A-granules because the B-granules failed to initiate. A population segregating for B-granule number was generated by crossing these two accessions and was used to study the genetic basis of B-granule initiation. A combination of Bulked Segregant Analysis and QTL mapping identified a major QTL located on the short arm of chromosome 4S that accounted for 44.4% of the phenotypic variation. The lack of B-granules in polyploid Aegilops with diverse genomes suggests that the B-granule locus has been lost several times independently during the evolution of the Triticeae. It is proposed that the B-granule locus is susceptible to silencing during polyploidization and a model is presented to explain the observed data based on the assumption that the initiation of B-granules is controlled by a single major locus per haploid genome.
登录
查看更多内容
影响因子：
3.1
作者：
Friebe, B;Tuleen, NA;Gill, BS
通讯作者：
Gill, BS
影响因子：
7
作者：
Nelson, W;Soderlund, C;Jackson, S
通讯作者：
Jackson, S
影响因子：
7.2
作者：
Roldan, Isaac;Wattebled, Fabrice;Merida, Angel
通讯作者：
Merida, Angel
影响因子：
7.2
作者：
Burton, RA;Jenner, H;Denyer, K
通讯作者：
Denyer, K
DOI：
10.1073/pnas.0305920101
发表时间：
2004-02-17
影响因子：
11.1
作者：
Bustos, R;Fahy, B;Smith, AM
通讯作者：
Smith, AM