Reproducibility of cine displacement encoding with stimulated echoes (DENSE) cardiovascular magnetic resonance for measuring left ventricular strains, torsion, and synchrony in mice
Reproducibility of cine displacement encoding with stimulated echoes (DENSE) cardiovascular magnetic resonance for measuring left ventricular strains, torsion, and synchrony in mice
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DOI：
10.1186/1532-429x-15-71
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发表时间：
2013-08-27
影响因子：
6.4
通讯作者：
Fornwalt, Brandon K.
中科院分区：
文献类型：
--
作者：
Haggerty, Christopher M.;Kramer, Sage P.;Fornwalt, Brandon K.
背景：先进的心功能指标因其优越的诊断和预测能力而在临床评估中日益重要。电影高密度心血管磁共振(CMR)基于其高空间分辨率和简化的后处理，是量化先进的心脏功能措施的理想选择。虽然许多研究已经在人类和小动物模型中使用了电影密度，但用于量化小鼠高级心功能的试验间和观察者间的重复性尚未得到评估。这对理解这项技术的能力和设计未来的实验来说都是一个关键的知识缺口。我们假设，Cine高密度CMR将在小鼠左心功能的高级测量中显示出良好的测试间和观察者间的重复性。方法：在7T ClinScan磁共振系统上，对5只正常小鼠(C57BL/6)和4只心功能低下(饮食诱导肥胖)的小鼠进行了两次成像，每隔两天进行一次成像。图像采集采用3个短轴方向(基轴、中轴、心尖)和2个长轴方向(4腔、2腔)，每个心动周期15~20帧。对左心室应变、扭转、扭转和同步性测量进行量化。两天的图像由一名观察员分析，以量化测试间的重复性，而观察者间的重复性则通过第二名观察员对第一天图像的分析来评估。结果：无论是观察者间还是试验间，变异系数(CoV)均具有较高的重复性
Background: Advanced measures of cardiac function are increasingly important to clinical assessment due to their superior diagnostic and predictive capabilities. Cine DENSE cardiovascular magnetic resonance (CMR) is ideal for quantifying advanced measures of cardiac function based on its high spatial resolution and streamlined post-processing. While many studies have utilized cine DENSE in both humans and small-animal models, the inter-test and inter-observer reproducibility for quantification of advanced cardiac function in mice has not been evaluated. This represents a critical knowledge gap for both understanding the capabilities of this technique and for the design of future experiments. We hypothesized that cine DENSE CMR would show excellent inter-test and inter-observer reproducibility for advanced measures of left ventricular (LV) function in mice.Methods: Five normal mice (C57BL/6) and four mice with depressed cardiac function (diet-induced obesity) were imaged twice, two days apart, on a 7T ClinScan MR system. Images were acquired with 15-20 frames per cardiac cycle in three short-axis (basal, mid, apical) and two long-axis orientations (4-chamber and 2-chamber). LV strain, twist, torsion, and measures of synchrony were quantified. Images from both days were analyzed by one observer to quantify inter-test reproducibility, while inter-observer reproducibility was assessed by a second observer's analysis of day-1 images. The coefficient of variation (CoV) was used to quantify reproducibility.Results: LV strains and torsion were highly reproducible on both inter-observer and inter-test bases with CoVs