Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway.
Ginkgolide C Suppresses Adipogenesis in 3T3-L1 Adipocytes via the AMPK Signaling Pathway.
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DOI：
10.1155/2015/298635
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Huang WC
中科院分区：
文献类型：
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作者：
Liou CJ;Lai XY;Chen YL;Wang CL;Wei CH;Huang WC
银杏内酯 C 是从银杏叶中分离出来的一种黄酮，据报道具有多种生物功能，包括降低血小板聚集和改善阿尔茨海默病。 本研究的目的是评估银杏内酯 C 在 3T3-L1 脂肪细胞中的抗脂肪形成作用。银杏内酯C用于处理分化的3T3-L1细胞。收集细胞上清液以测定甘油释放，并裂解细胞以分别通过蛋白质印迹和实时PCR测量与脂肪形成和脂肪分解相关的蛋白质和基因表达。银杏内酯 C 显着抑制分化脂肪细胞中的脂质积累。它还降低了脂肪生成相关转录因子的表达，包括过氧化物酶体增殖物激活受体和 CCAAT/增强子结合蛋白。此外，银杏内酯 C 增强脂肪甘油三酯脂肪酶和激素敏感性脂肪酶的产生，以促进脂肪分解，并增加 AMP 激活蛋白激酶 (AMPK) 的磷酸化，导致脂肪酸合成的乙酰辅酶 A 羧化酶活性降低。在与 AMPK 抑制剂（化合物 C）共培养时，银杏内酯 C 还可以改善分化的 3T3-L1 细胞中 Sirtuin 1 的激活和 AMPK 的磷酸化。结果表明，银杏内酯 C 是一种有效的黄酮，可通过激活的 AMPK 途径增加脂肪分解并抑制脂肪细胞中的脂肪生成。
Ginkgolide C, isolated from Ginkgo biloba leaves, is a flavone reported to have multiple biological functions, from decreased platelet aggregation to ameliorating Alzheimer disease. The study aim was to evaluate the antiadipogenic effect of ginkgolide C in 3T3-L1 adipocytes. Ginkgolide C was used to treat differentiated 3T3-L1 cells. Cell supernatant was collected to assay glycerol release, and cells were lysed to measure protein and gene expression related to adipogenesis and lipolysis by western blot and real-time PCR, respectively. Ginkgolide C significantly suppressed lipid accumulation in differentiated adipocytes. It also decreased adipogenesis-related transcription factor expression, including peroxisome proliferator-activated receptor and CCAAT/enhancer-binding protein. Furthermore, ginkgolide C enhanced adipose triglyceride lipase and hormone-sensitive lipase production for lipolysis and increased phosphorylation of AMP-activated protein kinase (AMPK), resulting in decreased activity of acetyl-CoA carboxylase for fatty acid synthesis. In coculture with an AMPK inhibitor (compound C), ginkgolide C also improved activation of sirtuin 1 and phosphorylation of AMPK in differentiated 3T3-L1 cells. The results suggest that ginkgolide C is an effective flavone for increasing lipolysis and inhibiting adipogenesis in adipocytes through the activated AMPK pathway.