Custom Mentholation of Commercial Cigarettes for Research Purposes.
Custom Mentholation of Commercial Cigarettes for Research Purposes.
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DOI：
10.1016/j.toxrep.2014.10.009
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Buehler, Stephanie S
中科院分区：
文献类型：
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作者：
MacGregor, Ian C;Stanfill, Stephen B;Gordon, Sydney M;Turner, Douglas J;Butler, Jenny M;Hanft, Elizabeth A;Kim, Hyoshin;Kroeger, Robyn R;Brinkman, Marielle C;Tefft, Margaret E;Clark, Pamela I;Buehler, Stephanie S
我们开发了一种简单且可重复的技术来创造只有薄荷醇含量不同的研究香烟。我们鉴定了一种可以定量测定香烟中薄荷醇和尼古丁含量的方法。我们调查了我们的定制薄荷醇香烟在储存过程中薄荷醇的损失率。我们目前正在人体暴露研究中使用这些定制的薄荷醇香烟。我们的定制薄荷醇香烟将有助于阐明薄荷醇对烟草烟雾毒性的影响。在美国，薄荷醇仍然是唯一被允许的卷烟香精添加剂，部分原因是薄荷醇香烟使用与烟草相关疾病风险增加之间的联系尚无定论。要进行确定性研究，需要只有薄荷醇含量不同的香烟，但这些香烟不能在商业上买到。我们将L-薄荷醇蒸气直接沉积到市售的非薄荷醇香烟上，制备了只有薄荷醇含量不同的研究香烟，并开发了一种测量香烟薄荷醇和尼古丁含量的方法。通过我们的定制薄荷醇技术，我们获得了所需的中等高薄荷醇含量(与商业品牌相比)，为6.7±1.0 mg/g(n=25)，而不影响香烟的尼古丁含量(17.7±0.7 mg/g[n=25])。我们还对我们的定制薄荷醇香烟的其他相关属性进行了表征，包括薄荷醇和尼古丁向主流烟雾的透过率，以及薄荷醇在室温下储存期间随时间的损失率。我们目前正在使用这种简单的薄荷醇技术来研究仅由于添加薄荷醇的存在而导致人类接触香烟烟雾中选定的化学物质的差异。我们的香烟还将有助于阐明薄荷醇对烟草烟雾毒性的影响。
We developed a simple and repeatable technique to create research cigarettes that differ only in menthol content. We qualified a method by which the menthol and nicotine content of a cigarette can be quantified. We investigated the rate of loss of menthol from our custom-mentholated cigarettes over time during storage. We are currently using these custom-mentholated cigarettes in human exposure studies. Our custom-mentholated cigarettes will help to elucidate the effects of menthol on the toxicity of tobacco smoke. In the U.S. menthol remains the sole permitted characterizing cigarette flavor additive in part because efforts to link menthol cigarette use to increased tobacco-related disease risk have been inconclusive. To perform definitive studies, cigarettes that differ only in menthol content are required, yet these are not commercially available. We prepared research cigarettes differing only in menthol content by deposition of l-menthol vapor directly onto commercial nonmenthol cigarettes, and developed a method to measure a cigarette's menthol and nicotine content. With our custom-mentholation technique we achieved the desired moderately high menthol content (as compared to commercial brands) of 6.7 ± 1.0 mg/g (n = 25) without perturbing the cigarettes’ nicotine content (17.7 ± 0.7 mg/g [n = 25]). We also characterized other pertinent attributes of our custom-mentholated cigarettes, including percent transmission of menthol and nicotine to mainstream smoke and the rate of loss of menthol over time during storage at room temperature. We are currently using this simple mentholation technique to investigate the differences in human exposure to selected chemicals in cigarette smoke due only to the presence of the added menthol. Our cigarettes will also aid in the elucidation of the effects of menthol on the toxicity of tobacco smoke.